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Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP12254-pAAV-AiE0459m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2254)
Plasmid#164454PurposeAiE0459m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neuronsDepositorInsertSYFP2
UseAAVPromoterminBetaGlobinAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
ExpressionBacterialAvailable SinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133639
Plasmid#152344PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;3xFlagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable SinceJune 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
RCAS-Flag-BRAFV600E
Plasmid#126615PurposeRetroviral expression of Flag tagged human oncogenic BRAFV600EDepositorAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NES
Plasmid#118939PurposeUsed to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
ExpressionPlantAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-BclXL-Blasticidin
Plasmid#120718PurposeExpresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertBl2-like protein 1, isoform Bcl-X(L)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutation33 c-terminal amino acidsPromoterCMVAvailable SinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2-gRNA-hyg-MCS
Plasmid#107734PurposeE. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.DepositorInsertX-3 site gRNA expression cassettes
UseCRISPRExpressionYeastPromoterSNR52pAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87919PurposeHIV-1 SIN lentiviral transfer vectorDepositorInsertsgHTT1, shGFP2, sgCas9, cherry
UseLentiviralPromoterH1, U6, 7SK, PGKAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1-N1011
Plasmid#92320PurposeN-term 1-1011 of HCF-1 proteinDepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(150-599)
Plasmid#90108PurposeMammalian expression of Flag-MCPIP1(150-599)DepositorAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1D_HUMAN_D0
Plasmid#79723PurposeThis plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pmirGLO-Smad5-1719
Plasmid#49382PurposeInsert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.DepositorInsertSmad5 (Smad5 Human, Mouse)
UseLuciferasePromoterHuman phosphoglycerate kinase (PGK) promoterAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-75-2
Plasmid#73036PurposeExpress an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cellsDepositorInsertp75-2
UseLentiviralExpressionMammalianMutationMature Human NT-3 gene exchanged Aspartic 15 to a…Available SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema5a-AP-His
Plasmid#72035PurposeExpresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Unc5b.a-Fc-His
Plasmid#72178PurposeExpresses the extracellular region of the Unc5B, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ncam1_1.18.1-AP-His
Plasmid#71962PurposeExpresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrp1-AP-His
Plasmid#71971PurposeExpresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only