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siRNA-resistant ABHD16A-Halo

Plasmid

#195163

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_2FLAG-AKAP79ΔCN

Plasmid

#183451

Purpose

Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

Depositor

Matthew Gold

Article

Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

Insert

AKAP5 (AKAP5 Human)

Tags

2xFLAG

Expression

Mammalian

Mutation

aa337-343 deleted

Promoter

CMV

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT221

Plasmid

#134889

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT222

Plasmid

#134890

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT202

Plasmid

#134879

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

deletion of Y208

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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p286 TIR1_HA_hH11

Plasmid

#164853

Purpose

constitutive TIR1 expression vector for human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

Insert

OsTIR1

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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PacrAB-acrAB-rfp + PmutS-mutS-yfp

Plasmid

#121443

Purpose

plasmid expressing translational fusions of AcrAB and MutS

Depositor

Mary Dunlop

Article

El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

Inserts

PmutS-mutS-yfp
PacrAB-acrAB-rfp

Use

Synthetic Biology; Pbb vector

Expression

Bacterial

Promoter

PacrAB and PmutS

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

Plasmid

#116885

Purpose

FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

Depositor

Brian Kuhlman

Article

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RVH)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mYFP

Plasmid

#105144

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mYFP

Tags

81nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mYFP

Plasmid

#105141

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mYFP

Tags

41nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-FNTA

Plasmid

#86859

Purpose

To express FNTA with a Venus tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

Venus

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSGE_763_4xUAS_UPS_hkCP

Plasmid

#71171

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

763_4xUAS_UPS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSGE_764_4xUAS_DOS_hkCP

Plasmid

#71172

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

764_4xUAS_DOS_hkCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_MC19_Trl-2xUAS_dCP

Plasmid

#71170

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MC19_Trl-2xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pJW1517

Plasmid

#62387

Purpose

pFA6a Osm1(M33A)-yoEGFP::LEU2

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

OSM1-EGFP fusion

Tags

EGFP

Expression

Yeast

Mutation

Methionine 33 mutated to Alanine

Promoter

Adh1p

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP140

Plasmid

#21672

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pFP120

Plasmid

#21673

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

LacZ (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, a 918…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pLIA EnR (CC#29)

Plasmid

#15152

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

Engrailed repressor (en Fly)

Use

Retroviral

Expression

Mammalian

Mutation

engrailed repressor domain

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pLIA CRX EnR (CC#145)

Plasmid

#15155

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Tags

Engrailed Repressor

Expression

Mammalian

Mutation

amino acids 7–108, containing the CRX homeobox

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only