We narrowed to 15,730 results for: NOL

1. AA401

   Plasmid

   #215959

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA048

   Plasmid

   #216016

   Purpose

   Fragmid fragment: (Cas protein) deactivated Cas

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dCas12a (D908A)_v1.1 [EnAs]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDB_131

   Plasmid

   #216095

   Purpose

   Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   CD97, CD4, CD26, CD274 guides
   

   Use

   CRISPR and Lentiviral; Positive controls

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_816

   Plasmid

   #216098

   Purpose

   CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas12a [EnAs]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1-Cdc45

   Plasmid

   #208796

   Purpose

   Xenopus laevis FLAG5-His10-TEV-Cdc45

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   Cdc45 (cdc45.L Frog)
   

   Tags

   FLAG and His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-DONSON-WM

   Plasmid

   #208798

   Purpose

   Xenopus laevis His6-Myc-DONSON (W234L, M463T)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   W234L; M463T

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-DONSON-S437A

   Plasmid

   #208803

   Purpose

   Xenopus laevis His6-Myc-DONSON (S437A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   S437A

   Promoter

   T7

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-DONSON-ΔD1

    Plasmid

    #208804

    Purpose

    Xenopus laevis His6-DONSON (1-155aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    ΔD1, deletion of 1-155aa

    Promoter

    T7

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. sfGFP-SasG(3G)-mCherry

    Plasmid

    #206945

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (3G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (3G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLINE1ΔORF2-scFvFc

    Plasmid

    #206021

    Purpose

    The ORF2 sequence was removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
    

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLINE1ΔORF1-2-scFvFc

    Plasmid

    #206022

    Purpose

    The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. sfGFP-SasG(7G)-mCherry

    Plasmid

    #206949

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (7G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (7G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. sfGFP-SasG(6G)-mCherry

    Plasmid

    #206948

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (6G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (6G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sfGFP-SasG(5G)-mCherry

    Plasmid

    #206947

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (5G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (5G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sfGFP-SasG(4G)-mCherry

    Plasmid

    #206946

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (4G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (4G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sfGFP-SasG(2G)-mCherry

    Plasmid

    #206944

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (2G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (2G)-mCherry
    

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc

    Plasmid

    #200127

    Purpose

    Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)]-Fluc

    Plasmid

    #200128

    Purpose

    Retroviral expression of SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(SA)] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[CoVA(SA)] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge