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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

Plasmid

#163027

Purpose

FLiCRE TF with fast kinetics for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

CD4-MKII-f-hLOV1-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-BE4-AmAPOBEC1

Plasmid

#138342

Purpose

Mammalian expression plasmid for BE4-AmABOBEC1

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-AmAPOBEC1

Use

Synthetic Biology

Tags

BP-NLS

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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LL - hNANOGi

Plasmid

#12196

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

NANOG - RNAi (NANOG Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P29

Plasmid

#120040

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-II

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Strep-II

Use

Pcr template

Tags

Strep-II

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-Gal4

Plasmid

#62899

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P19

Plasmid

#124789

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFW6000

Plasmid

#224215

Purpose

Bacteroides genomic editing tool; aTc inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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LEPZ

Plasmid

#111161

Purpose

miR-E (miR-30 variant)-based RNAi with ZsGreen reporter

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Retroviral

Mutation

WT

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pPyCAG-mKate2-3xNLS-IRES-Pac

Plasmid

#183618

Purpose

Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

Insert

mKate2-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-PIPK

Plasmid

#96810

Purpose

cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-PIPK

Tags

YFP

Expression

Mammalian

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFH2.30_Gag-MCP-dpol-T2A-eGFP

Plasmid

#205538

Purpose

Express HIV Gag-MCP-dpol-T2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

HIV Gag-MCP-dpol

Expression

Mammalian

Mutation

pol frameshift sequence recoded

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-3xrGBD

Plasmid

#176100

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Brn2

Plasmid

#32925

Purpose

Retroviral expression of mouse Brn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pCAGGs-Foxp1

Plasmid

#45720

Depositor

Jeremy Dasen

Article

Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

Insert

FoxP1 (Foxp1 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-BE4-RrA3F

Plasmid

#138340

Purpose

Mammalian expression plasmid for BE4-RrA3F

Depositor

Nicole Gaudelli

Article

Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

Insert

BE4-RrA3F

Tags

BP-NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pKM3

Plasmid

#154168

Purpose

Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Type

Empty backbone

Expression

Insect

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Zeo

Plasmid

#227261

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-TJP1

Plasmid

#227299

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TJP1 (TJP1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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