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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,569 results for: fus

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Showing: 8921 - 8940 of 10569 results

  5. Venus-7aa-MAO-pEGFP-C3

    Plasmid
    #166765
    Purpose
    To target Venus to the mitochondrial outer membrane. MAO tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    MAO tail anchor (MAOA Human)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    166765-plasmid-map-sequence-id-328859

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  6. Venus-7aa-Cb5-pEGFP-C1

    Plasmid
    #166764
    Purpose
    To target Venus to the Endoplasmic Reticulum. Cb5 tail anchor inserts into the membrane and Venus faces the cytosol. This serves as a collisional control in FLIM-FRET experiments.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5a Rat)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166764-plasmid-map-sequence-id-328853

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  9. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  10. pMT3-RhoGC E254D

    Plasmid
    #100033
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.
    Depositor
    Daniel Oprian
    Article
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC E254D
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    E254D
    Promoter
    Adenovirus major late promoter
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid
    #86933
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86933-plasmid-map-sequence-id-174748

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  16. pPD2X

    Plasmid
    #86967
    Purpose
    Expresses PCNA2 protein fused phosphite dehydrogenase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Watanabe et al ChemCatChem 2013 Dec;5(12):3835-3840
    Insert
    PCNA2 fused to phosphite dehydrogenase and putidaredoxin
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, C73S/C85S in PdX
    Promoter
    T7 promoter
    Available Since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86967-plasmid-map-sequence-id-173938

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  18. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

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  19. JA1290

    Plasmid
    #49968
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-9 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. JA1261

    Plasmid
    #49963
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 8921 - 8940 of 10569 results