We narrowed to 8,486 results for: Pet2

1. pET21-pelB-GST-2

   Plasmid

   #228984

   Purpose

   For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-2
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-GST-1

   Plasmid

   #228983

   Purpose

   For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaTdT-1

   Plasmid

   #228974

   Purpose

   For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaTdT-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21-pelB-LaRIgG-7

   Plasmid

   #228997

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-LaRIgG-1

   Plasmid

   #228995

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-1
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21-pelB-LaMIgG-5

   Plasmid

   #228991

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaMIgG-5
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21 gamma-tubulin

   Plasmid

   #101825

   Purpose

   production of gamma-tubulin in bacteria

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Whole protein

   Promoter

   T7

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21a-LysRS-His

   Plasmid

   #124114

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Lysine-tRNA ligase (lysU Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a-AspRS-His

   Plasmid

   #124106

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Aspartate-tRNA ligase (aspS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-EGFP-CNA35

    Plasmid

    #61603

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with EGFP

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    EGFP and CNA35
    

    Use

    Tags

    His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-HaloTag-CNA35

    Plasmid

    #215457

    Purpose

    Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    CNA35
    

    Use

    Tags

    HaloTag

    Expression

    Bacterial

    Mutation

    Includes stop codon

    Promoter

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-EGFP-CNA35

    Plasmid

    #61603

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with EGFP

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    EGFP and CNA35
    

    Use

    Tags

    His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a-ProRS-His

    Plasmid

    #124117

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Prolyl-tRNA ligase (proS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21a-ValRS-His

    Plasmid

    #124122

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Valyl-tRNA ligase (valS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-HaloTag-CNA35

    Plasmid

    #215457

    Purpose

    Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

    Depositor

    Johanna Ivaska

    Article

    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

    Insert

    CNA35
    

    Use

    Tags

    HaloTag

    Expression

    Bacterial

    Mutation

    Includes stop codon

    Promoter

    Available since

    May 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pET21a-alpha-synuclein

    Plasmid

    #51486

    Purpose

    Inducible expression of human alpha-synuclein in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pET28a-Cas9-His

    Plasmid

    #98158

    Purpose

    Purification of Cas9 protein

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

    Insert

    NLS-Cas9-NLS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21b Hot-PETase

    Plasmid

    #225761

    Purpose

    Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    Hot-PETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-Cas9-His

    Plasmid

    #98158

    Purpose

    Purification of Cas9 protein

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

    Insert

    NLS-Cas9-NLS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21b Hot-PETase

    Plasmid

    #225761

    Purpose

    Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    Hot-PETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Jin Wang, Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET29b_1_anti-GFP-nanobody

    Plasmid

    #210394

    Purpose

    Express anti-GFP nanobody in E. coli

    Depositor

    Magnus Rueping

    Article

    Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

    Insert

    1_anti-GFP-nanobody
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Jin Wang, Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET21a-Streptavidin-Alive

    Plasmid

    #20860

    Purpose

    Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

    Insert

    Streptavidin-Alive
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Wt gene

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET24a-VHH-myc

    Plasmid

    #109422

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET24a-VHH-APEX2

    Plasmid

    #109420

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET263-pUC57 24xMS2V7

    Plasmid

    #140705

    Purpose

    MS2V7 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V7
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    24 MS2 loops V7, high-affinity C-variant, inter-s…

    Promoter

    None

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28-his-3C_Tau352

    Plasmid

    #100092

    Purpose

    Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

    Depositor

    Nikolai Sluchanko

    Article

    Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

    Insert

    Fetal microtubule-associated protein tau (MAPT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    wild type sequence

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge