We narrowed to 2,070 results for: gem
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Plasmid#138167PurposeExpresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
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promoter J23110 (upstream of RBS, mRFP1)
Plasmid#117852PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertmedium-high promoter, RBS, mRFP1
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZeo-Cas9-gate
Plasmid#113744PurposeGateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.DepositorInsertCas9
UseCRISPR and Gateway CloningPromoterZ. Mays ubiquitin promoter, 35S promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-Akt2(T309A)
Plasmid#86625PurposeExpresses eGFP-tagged human Akt2(T309A) phosphomutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt2(T309A) (AKT2 Human)
ExpressionMammalianAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPML1 (GB0045)
Plasmid#68164PurposeProvides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid partDepositorInsertAtML1 promoter
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
tFucci(CA)5pPBbsr2
Plasmid#197351PurposeEncoding tFucci(CA)5.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttFucci(CA)5
TagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionMammalianAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-ColH-HylB
Plasmid#248631Purposeexpresses Myc-tagged ColH and His-tagged HylB as two separate proteins in E. coliDepositorInsertMyc-tagged bacterial collagenase, ColH, and His-tagged hyaluronidase, HylB
TagsHis and MycExpressionBacterialPromoteraraBADAvailable sinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PVT1-luciferase
Plasmid#113350Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPVT1-enhancer (PVT1 Human)
UseLuciferaseAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(D323A/D325A)
Plasmid#86630PurposeExpresses eGFP-tagged human Akt1(D323A/D325A) mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAkt1(D323A/D325A) (AKT1 Human)
ExpressionMammalianAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
px330-GAPDH
Plasmid#136940PurposeNHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting GAPDH (GAPDH Human)
UseCRISPRExpressionMammalianAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
px330-CD4
Plasmid#136938PurposeNHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDHDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting CD4 (CD4 Human)
UseCRISPRExpressionMammalianAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
Tags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…Available sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJC5-4-insulator-BREreporter-insulator
Plasmid#171499Purposeinjections to make Bmp reporter mouse transgenic lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInsulator BRE-MLP:H2B-Turqoise
ExpressionMammalianPromoterBRE-MLPAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU1
Plasmid#72934PurposeBackbone vector with internal BsaI removed by site-directed mutagenesisDepositorPublicationTypeEmpty backboneUseSynthetic BiologyAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRTCLCrVB1.3
Plasmid#22844DepositorInsertB DNA
UseCotton vigs vectorExpressionBacterialMutation1.3 unit B componet length inserted into pBlueScr…Available sinceMarch 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
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BBa_J72110-BBa_J72094
Plasmid#40778DepositorInsertAraC-pBad + coi
UseSynthetic BiologyExpressionBacterialAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
XP3 Xwnt-4
Plasmid#16988DepositorInsertXwnt-4 (wnt4 Frog)
ExpressionBacterialAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT33
Plasmid#89668Purposeexpression of doubleTAL staple protein dT33 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT33
TagsHis6ExpressionBacterialPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -