We narrowed to 1,045 results for: hug;

1. NYESO alpha into TRAC HDRT Source (pTR 223)

   Plasmid

   #112023

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO alpha into TRAC HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NYESO beta into TRBC1 HDRT Source (pTR 277)

   Plasmid

   #112024

   Purpose

   DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   NYESO beta into TRBC1 HDRT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV[TetOn]-Puro-TRE3G>mNeurog2

   Plasmid

   #198754

   Purpose

   Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Neurogenin-2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV[TetOn]-Puro-TRE3G>mAscl1

   Plasmid

   #198755

   Purpose

   Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sabatini/Lander Lab Ras synthetic lethal candidate focused human sgRNA library in lentiCRISPRv1

   Pooled Library

   #92352

   Purpose

   contains 6,661 sgRNAs (comprising 499 intergenic control sgRNAs and 6,162 sgRNAs targeting 132 control and candidate Ras synthetic lethal genes at up to 50 sgRNAs per gene)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Species

   Homo sapiens

   Available since

   June 7, 2017

   Availability

   Academic Institutions and Nonprofits only

6. RAB11A-GFP HDRT Source (pTR 143)

   Plasmid

   #112012

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   RAB11A-GFP HDRT (RAB11A Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RAB11A-mCherry HDRT Source (pTR 144)

   Plasmid

   #112013

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   RAB11A-mCherry HDRT (RAB11A Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CD4-C-GFP HDRT Source (pTR 152)

   Plasmid

   #112018

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CD4-GFP HDRT (CD4 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CLTA-GFP HDRT Source (pTR 153)

   Plasmid

   #112016

   Purpose

   DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP

   Depositor

   Alexander Marson

   Article

   Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

   Insert

   CLTA-GFP HDRT (CLTA Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CLTA-mCherry HDRT Source (pTR 177)

    Plasmid

    #112017

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CLTA-mCherry HDRT (CLTA Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BATF-GFP HDRT Source (pTR 146)

    Plasmid

    #112015

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    BATF-GFP HDRT (BATF Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RAB11A-BFP HDRT Source (pTR 145)

    Plasmid

    #112014

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-BFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CD4-C-BFP HDRT Source (pTR 176)

    Plasmid

    #112020

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-BFP HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CD4-C-mCherry HDRT Source (pTR 175)

    Plasmid

    #112019

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    CD4-mCherry HDRT (CD4 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PG13-mCherry

    Plasmid

    #195291

    Purpose

    mCherry reporter containing 13 copies of the p53-binding consensus sequence

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUB2.1

    Plasmid

    #40728

    Purpose

    Modified pcDNA3.1 vector for ligation independent cloning, including a ccdB cassette for eliminating no-insert background

    Depositor

    Thomas Hughes

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cre Shine

    Plasmid

    #37404

    Purpose

    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.

    Depositor

    Thomas Hughes

    Insert

    Cre Shine
    

    Use

    Cre/Lox

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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