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Plasmid#216281PurposeThree affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate CatcherDepositorInsertSnoopTag-SpyTag-(AffiHER2)x3
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, and SpyTagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24-95~236
Plasmid#208634Purposefor E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236DepositorInserthuman RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationonly aa 95~236, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:em-blue-shift.1
Plasmid#191928PurposeEncodes a GFP design, sf:em-blue-shift.1DepositorInsertsf:em-blue-shift.1
UseTagsHis TagExpressionBacterialMutationT65S Q69P S72A T108V Y145M T167V V224IPromoterT7Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED 4CA-FLAG
Plasmid#199681PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-FlagDepositorInsertLeucine zipper LYCHOS LED 4CA (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationC595.604.629.638 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a LZ LED Y551A-FLAG
Plasmid#199680PurposeBacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-FlagDepositorInsertLeucine zipper LYCHOS LED Y551A (GPR155 Human)
UseTags6xHis and FlagExpressionBacterialMutationY551 of LYCHOS mutated to APromoterT7 promoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmAGO1-RC_F
Plasmid#146262PurposeBacterial Expression of DmAGO1-RCDepositorInsertDmAGO1-RC (AGO1 Fly)
UseTagsExpressionBacterialMutationone non silent mutation R787C compared to sequenc…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmGW182_1-539_F
Plasmid#146263PurposeBacterial Expression of DmGW182_1-539DepositorInsertDmGW182_1-539 (gw Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28 sf:em-blue-shift.2
Plasmid#191919PurposeEncodes a GFP design, sf:em-blue-shift.2DepositorInsertsf:em-blue-shift.2
UseTagsHis TagExpressionBacterialMutationT65S S72A T108I Y145F V150IPromoterT7Available sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)-MutTER
Plasmid#178035PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deleted and Changed T(8…PromoterT7LacIAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-amylase sensor
Plasmid#186293PurposeExpression alpha-amylase sensor in e coliDepositorInsertcpLumiLuc-amylase sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET28a-cpLumiLuc-HSA sensor
Plasmid#186294PurposeExpression Human serum albumin sensor in e coliDepositorInsertcpLumiLuc-HSA sensor
UseTagsHisTagExpressionBacterialMutationPromoterAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-1-H340D_enzymatic-domain
Plasmid#183388PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1 (AA130-404)
UseTagsHis-TEVExpressionBacterialMutationH340DPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_canine-Caspase-1/4_enzymatic-domain
Plasmid#183383PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E60amb)-His
Plasmid#168043PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 60 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop C
Plasmid#171781PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-DogTag Loop C
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop B
Plasmid#171777PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-SpyTag003 Loop B
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop C
Plasmid#171778PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-SpyTag003 Loop C
UseTagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only