167,895 results

1. Spectrin-M-cpstFRET

   Plasmid

   #61109

   Purpose

   Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

   Depositor

   Fred Sachs

   Article

   Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

   Insert

   spectrin-cpstFRET
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. TK4-NLS-EGFP

   Plasmid

   #239046

   Purpose

   PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

   Depositor

   Marius Wernig

   Inserts

   EGFP
   puroR-T2A-mycNLS-mTagBFP2
   

   Tags

   T2A-mycNLS-mTagBFP2 and c-myc-NLS

   Expression

   Mammalian

   Promoter

   CAG and EF1a

   Available Since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human V5-IRF3-pcDNA3

   Plasmid

   #32713

   Depositor

   Saumen Sarkar

   Article

   Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

   Insert

   IRF3 (IRF3 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLB1080

   Plasmid

   #37826

   Depositor

   Ann Hubbard

   Article

   Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

   Insert

   ATP7B (ATP7B Human)
   

   Tags

   mEGFP

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

   Viral Prep

   #92379-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Available Since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

7. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pT3TS-RfxCas13d-NLS-HA

   Plasmid

   #141321

   Purpose

   Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d (NLS-RfxCas13d-NLS)
   

   Use

   CRISPR

   Tags

   HA and NLS

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET12a-PAPI

   Plasmid

   #19961

   Depositor

   Jonathan Tait

   Article

   Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

   Insert

   Annexin V (ANXA5 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. ND4-DdCBE-right side TALE

    Plasmid

    #157843

    Purpose

    expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. T7RNAP Cyslight

    Plasmid

    #205077

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Tags

    6xHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAdx-CMV-copGFP

    Plasmid

    #73346

    Purpose

    Express copGFP under CMV promoter

    Depositor

    Kazuhiro Oka

    Insert

    CMV-copGFP
    

    Use

    Adenoviral

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 luciferase

    Plasmid

    #26612

    Depositor

    Jie Chen

    Article

    Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

    Insert

    firefly luciferase
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pWPI-LUC7L2-3xFLAG

    Plasmid

    #201641

    Purpose

    Over-expression of 3xFLAG-tagged human LUC7L2

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L2 (LUC7L2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRE112

    Plasmid

    #43828

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (AAV5)

    Viral Prep

    #121539-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (#121539). In addition to the viral particles, you will also receive purified pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) plasmid DNA. Synapsin-driven, HA-tagged hM3D(Gq) for neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Christopher Richie

    Article

    Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

    Promoter

    Syn

    Tags

    HA

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only