Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 73,554 results for: LAS

Showing: 8981 - 9000 of 73554 results

  1. A-PRMT3-3

    Plasmid
    #166514
    Purpose
    scFv of a human scaffold targeting Protein arginine methyltransferase 3 isoform 1. Antigen coverage aa 211-531 of 531.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166514-plasmid-map-sequence-id-329056

    Enlarge

  2. Q-PHIPA-23

    Plasmid
    #166547
    Purpose
    scFv of a human scaffold targeting PH-interacting protein. Antigen coverage aa 1302 - 1434 of 1821, Bromodomain 2
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166547-plasmid-map-sequence-id-328319

    Enlarge

  3. C-BRD1-3

    Plasmid
    #166517
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 1. Antigen coverage aa 925-1049 of 1058, PWWP domain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166517-plasmid-map-sequence-id-328311

    Enlarge

  4. O-FHL1-1

    Plasmid
    #166543
    Purpose
    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166543-plasmid-map-sequence-id-328279

    Enlarge

  5. O-AASSA-25

    Plasmid
    #166537
    Purpose
    scFv of a human scaffold targeting Aminoadipate-semialdehyde synthase. Antigen coverage aa 455-926 of 926
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166537-plasmid-map-sequence-id-328275

    Enlarge

  6. pANY4-pR18/pL18-ccdB

    Plasmid
    #164899
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164899-plasmid-map-sequence-id-327990

    Enlarge

  7. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

    Enlarge

  8. pZSCAN18-donor

    Plasmid
    #113821
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFP
    Depositor
    Kevin White
    Insert
    ZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113821-plasmid-map-sequence-id-241248

    Enlarge

  9. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

    Enlarge

  10. pZNF561-donor

    Plasmid
    #113801
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF561 with GFP
    Depositor
    Kevin White
    Insert
    ZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113801-plasmid-map-sequence-id-229885

    Enlarge

  11. pZNF700-donor

    Plasmid
    #112385
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF700 with GFP
    Depositor
    Kevin White
    Insert
    ZNF700 homology arms flanking EGFP-IRES-Neo cassette (ZNF700 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112385-plasmid-map-sequence-id-229845

    Enlarge

  12. pZNF169-donor

    Plasmid
    #112376
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF169 with GFP
    Depositor
    Kevin White
    Insert
    ZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112376-plasmid-map-sequence-id-229740

    Enlarge

  13. pZFP1-donor

    Plasmid
    #112374
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFP1 with GFP
    Depositor
    Kevin White
    Insert
    ZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112374-plasmid-map-sequence-id-229736

    Enlarge

  14. pZNF227-donor

    Plasmid
    #112368
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF227 with GFP
    Depositor
    Kevin White
    Insert
    ZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112368-plasmid-map-sequence-id-229716

    Enlarge

  15. pOTX1-donor

    Plasmid
    #112364
    Purpose
    CRISPR donor plasmid to tag human transcription factor OTX1 with GFP
    Depositor
    Kevin White
    Insert
    OTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112364-plasmid-map-sequence-id-229691

    Enlarge

  16. pZNF175-donor

    Plasmid
    #112347
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF175 with GFP
    Depositor
    Kevin White
    Insert
    ZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112347-plasmid-map-sequence-id-229660

    Enlarge

  17. pZNF354C-donor

    Plasmid
    #112339
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354C with GFP
    Depositor
    Kevin White
    Insert
    ZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112339-plasmid-map-sequence-id-228576

    Enlarge

  18. pZNF347-donor

    Plasmid
    #112326
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF347 with GFP
    Depositor
    Kevin White
    Insert
    ZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112326-plasmid-map-sequence-id-228548

    Enlarge

  19. Mmadhc

    Plasmid
    #74662
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    Mmadhc (MMADHC Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pJK528

    Plasmid
    #71625
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes methionine-105 to serine, isoleucine-109 …
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    71625-plasmid-map-sequence-id-131668

    Enlarge

Showing: 8981 - 9000 of 73554 results