We narrowed to 10,569 results for: fus
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Plasmid#217735PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG4 (ADGRG4 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2727Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG
Plasmid#217733PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG2 (ADGRG2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-612Available SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_MYH10_CDKN2A
Plasmid#205864PurposeExpress mEGFP-tagged fusion protein, MYH10_CDKN2A from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CD44_PDHX
Plasmid#205790PurposeExpress mEGFP-tagged fusion protein, CD44_PDHX from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
V5-TurboID-Mfn1E209A
Plasmid#195156Purposemammalian expression of TurboID tagged Mfn1E209ADepositorInsertTurboID
TagsMfn1-E209AExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterCMVAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS2387-Tier1-PhCMV-p2a_mTagBFP2
Plasmid#169524PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).DepositorInsertPCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-79aa-ActA-pEGFP-C1
Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
TagsVenusExpressionMammalianPromoterCMVAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB6-Gfap-T2A-tdTomato-FNF-DTA
Plasmid#140271PurposeB6J Gfap-T2A-tdTomato allele targeting vector, low copy numberDepositorInsertGfap (Gfap Mouse)
UseMouse TargetingTagsC-terminal fusion of T2A-tdTomatoExpressionMammalianAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC2-NMM-tdtomato
Plasmid#129557PurposepClimDC-NMM:tdT cell membrane markerDepositorInsertN-Myristoylation motif (NMM) fused to tdtomato
ExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-xlH2B(1-114)_K114A
Plasmid#118217PurposeBacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.DepositorInsertxlH2B(1-114)
TagsMxe-GyrA intein fused to chitin binding domainExpressionBacterialMutationLysine 114 to alaninePromoterT7Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP251 pEF1a-UNC5C-4-SunTag
Plasmid#100940PurposeTALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO geneDepositorInsertTALE target sequence for UNC5C, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGH42
Plasmid#107249PurposeVipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).DepositorInsertvipp1-gfp
UseSuicide vector for destined for double homologous…TagsmGFPmut3ExpressionBacterialPromoternative promoter of sll0617Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
RGD-ACP-I27-CC
Plasmid#85391PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and ACP tag for fluorophore labelingDepositorInsertRGD motif for integrin binding fused to acyl carrier protein (ACP) tag and titin immunoglobulin domain (I27)
Tags6x HisExpressionBacterialPromoterT7Available SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWPT-UCU/ACC-mEGFP-IRES-mCherry
Plasmid#49232PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49219PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
SPL10p::pCGTAG (SPL10p::NLS-GFP)
Plasmid#27410DepositorInsertSPL10 promoter (AT1G27370 Mustard Weed)
MutationSPL10 promoter transcriptionally fused to a nucle…Available SinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
TagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(MGD) (SB264)
Plasmid#227613PurposeInducible expression of Strep-tagged Opa1(MGD) in E.coliDepositorInsertOPA1 (OPA1 Human)
TagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-580 (MGD) and aa 938-960PromoterT7Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-mouse cGAS-GFP
Plasmid#186877PurposeDoxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transductionDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG
Plasmid#217717PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR1 (CELSR1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2453Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only