We narrowed to 13,205 results for: lic

1. pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

   Plasmid

   #225490

   Purpose

   Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   Neuronal expression

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES2-eGFP:HA-mTREK-1(tandem S131C-S131C)

   Plasmid

   #224779

   Purpose

   Mammalian expression vector for generating a tandem mTREK-1 with two identical monomers linked through a 20 AA flexible linker

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Tandem TREK-1 S131C-S131C) (Kcnk2 Mouse)
   

   Expression

   Mammalian

   Mutation

   S131C / S562C

   Promoter

   CMV

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

   Plasmid

   #224755

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Mutation

   S131C/C159A/C219A/C365A/C399A

   Promoter

   T7

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

   Plasmid

   #224756

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Mutation

   S131A/C159A/C219A/C365A/C399A

   Promoter

   T7

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1295-AAV-EFSNC-dCjCas9-HP1a(72-177)

   Plasmid

   #223140

   Purpose

   Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1a (CBX5 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 72-177

   Promoter

   EF1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1305-AAV-EFSNC-dSaCas9-HP1a(72-177)

   Plasmid

   #223150

   Purpose

   Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   HP1a (CBX5 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 72-177

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

   Plasmid

   #223144

   Purpose

   Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD1 (MBD1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 529-592

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

   Plasmid

   #223154

   Purpose

   Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD1 (MBD1 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 529-592

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-mTet1s_K852R pc3914

   Plasmid

   #201704

   Purpose

   Expresses of GFP-tagged mTet1s_K852R in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s-K852R (Tet1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 852 mutated to arginine

   Promoter

   CAG

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGFP-mTet1s_K852E pc3915

    Plasmid

    #201705

    Purpose

    Expresses of GFP-tagged mTet1s_K852E in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s-K852E (Tet1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lysine 852 mutated to glutamate

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. JO-pH720-SARS-CoV-2-S-tail-WT

    Plasmid

    #218453

    Purpose

    Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

    Insert

    Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
    

    Tags

    eXact

    Expression

    Bacterial

    Available Since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCBE3-HF1

    Plasmid

    #218156

    Purpose

    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCBE3-HF1-Hypa

    Plasmid

    #218158

    Purpose

    This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCBE3-Hypa

    Plasmid

    #218157

    Purpose

    This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N692A…

    Promoter

    rpsL promoter

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV2_CAG_oROS-HT(C199S)_WPRE

    Plasmid

    #216415

    Purpose

    Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT(C199S)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid

    #208966

    Purpose

    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid

    #208973

    Purpose

    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiCRISPR v2-sgABI2-1

    Plasmid

    #210139

    Purpose

    knock out ABI2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Abl interactor 2 (ABI2 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR v2-sgCYFIP1-1

    Plasmid

    #210137

    Purpose

    knock out CYFIP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2-sgABI2-2

    Plasmid

    #210140

    Purpose

    knock out ABI2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Abl interactor 2 (ABI2 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge