We narrowed to 166,303 results for: addgene

1. pT7gRNA-ttn.1_C3

   Plasmid

   #140866

   Purpose

   sgRNA synthesis vector for ttn.1_C3 (zebrafish titin .1).

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   zebrafish ttn.1_C3 sgRNA for in vitro transcription (ttn.1 Synthetic, Zebrafish)
   

   Use

   In vitro transcription of sgrnas

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-EGxxFP-PIGA

   Plasmid

   #153958

   Purpose

   Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 401-bp fragment from intron 5 and exon 6)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine

   Plasmid

   #160069

   Purpose

   Conditionally overexpress collybistin (CB2SH3-) and mCitrine

   Depositor

   Angel de Blas

   Article

   George et al eNeuro. 2021 Jun 2. pii: ENEURO.0561-20.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   AAV

   Tags

   HA- in CB2SH3

   Promoter

   human Synapsin promoter

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTCO2-mTDGi.2

   Plasmid

   #149431

   Purpose

   miniTdg gene, SUMOylation mutant

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Insert

   Tdg (Tdg Mouse)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

   Promoter

   Tdg promoter

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTCO2-mTDGi.1

   Plasmid

   #149430

   Purpose

   miniTdg gene, catalytic mutant

   Depositor

   Primo Schaer

   Article

   Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

   Insert

   Tdg (Tdg Mouse)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   N151A catalytic mutant, SNPs of Tdg gene in OLA/1…

   Promoter

   Tdg promoter

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-Myosin7B-sgRNA

   Plasmid

   #154861

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   MYO7B sgRNA (MYO7B Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MEN2A-MAC

   Plasmid

   #139634

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   MEN2A (RET Human)
   

   Tags

   StrepIII-HA-BirA*

   Expression

   Mammalian

   Mutation

   C634W

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Y365A or A365Y inducible rescue

   Plasmid

   #131323

   Purpose

   gRNA to generate i-WT-r or i-MT-r systems

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   Y365A or A365Y inducible rescue
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTF2_KO_ex11_gRNA-4

   Plasmid

   #131334

   Purpose

   gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

   Depositor

   Danny Reinberg

   Article

   Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

   Insert

   MTF2_KO_ex11_gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LMX1B-gRNA-C-del

    Plasmid

    #131516

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-C-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

11. LMX1B-gRNA-N-del

    Plasmid

    #131333

    Purpose

    gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    LMX1B-gRNA-N-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HoxD del ds1

    Plasmid

    #131338

    Purpose

    gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EVX2-gRNA-C-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SKOR1-gRNA-C-del

    Plasmid

    #131332

    Purpose

    gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    SKOR1-gRNA-C-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SKOR1-gRNA-N-del

    Plasmid

    #131331

    Purpose

    gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    SKOR1-gRNA-N-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HoxD del control ds

    Plasmid

    #131336

    Purpose

    gRNA to delete control region near HoxD. Use with HoxD del control us

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    CTRL-gRNA-C-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

16. HoxD del us1

    Plasmid

    #131337

    Purpose

    gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EVX2-gRNA-N-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. HoxD del control us

    Plasmid

    #131335

    Purpose

    gRNA to delete control region near HoxD. Use with HoxD del control ds

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    CTRL-gRNA-N-del
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EED-KO-gRNA-1

    Plasmid

    #131321

    Purpose

    gRNA to knockout EED. Use with EED-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    EED-KO-gRNA-1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

19. Met133I, M1381I, M186I ELL2

    Plasmid

    #127263

    Purpose

    For in vitro translation of human ELL2 with Met133I, M1381I, M186I

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    Three Mets (133, 138, 186) to Ileu

    Promoter

    T7

    Available Since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128345

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrnb4 (Chrnb4 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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