We narrowed to 36,680 results for: kan
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Plasmid#171214PurposeGateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.DepositorInsertBNT162b2ΔUTR
UseGateway CloningMutationKozak sequence changed from CCCGCCACC to GGCTGCAC…Available SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC22A3_STOP
Plasmid#161190PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC22A3 (SLC22A3 Human)
ExpressionMammalianAvailable SinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC18A2_STOP
Plasmid#161163PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC18A2 (SLC18A2 Human)
ExpressionMammalianAvailable SinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC22A8_STOP
Plasmid#161155PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC22A8 (SLC22A8 Human)
ExpressionMammalianAvailable SinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221rAPOBEC1-T7RNAP
Plasmid#167979PurposeExpresses fusion protein rAPOBEC1-T7RNAP in E. coliDepositorInsertrAPOBEC1-T7RNAP (T7p07 Synthetic)
TagsT7 tag and T7RNAPExpressionBacterialPromoterTetR-PtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221T7RNAP
Plasmid#167974PurposeExpresses T7RNAP in E. coliDepositorInsertT7RNAP (T7p07 Synthetic)
PromoterTetR-PtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ6
Plasmid#167135PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterKanamycin promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2
Plasmid#166972PurposeExpresses FBF-2 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
TagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1 (273-427)
Plasmid#162734PurposeBacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1 domain residues 273-427
ExpressionBacterialMutationCodon optimisedAvailable SinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-CV-SNAP25
Plasmid#168031PurposeExpresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cellsDepositorInserteCFP-SNAP25(A141-G206)-mVenus
TagsHisx6ExpressionBacterialAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C-EP300
Plasmid#166454PurposeExpresses fusion of mCherry and EP300-C-IDRDepositorInsertmCherry, C-EP300(1795-2414) (EP300 Human)
ExpressionMammalianAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-TREM2-2
Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-tBid-pEGFP-C1
Plasmid#166736PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, tBidDepositorAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB_PVX (GB1464)
Plasmid#160612PurposePVX vector adapted to GBDepositorInsertPVX vector adapted to GB
UseSynthetic BiologyAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB301TOR-CRISPR
Plasmid#164797PurposeFor gene editing in oomycetesDepositorInsertsPsNLS-hSpCas9
sgRNA expression cassette
UseOomycete expressionPromoterHAM34 and RPL41Available SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIA1362
Plasmid#166860PurposeExpression of codon optimized SARS-COV-2 Nsp7 in E. coliDepositorAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC28-LIC-sfGFP-His
Plasmid#165479PurposepET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tagDepositorTypeEmpty backboneTagsTEV-GFP-6xHisExpressionBacterialAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS018E: Entry vector for: ratiometric pHluorin (mitchondrial)
Plasmid#163070PurposeEntry vector for: ratiometric pHluorin (mitchondrial)DepositorInsertratiometric pHluorin
UseGateway CloningAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBNG2
Plasmid#149473PurposeBroad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertNahR(AM)-GFP
UseSynthetic BiologyExpressionBacterialPromoterPSalTTCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only