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Showing: 9061 - 9080 of 66847 results

  1. pCMV6-AC 4-μHOM

    Plasmid
    #113619
    Purpose
    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP
    Depositor
    Jeremy Stark
    Publication
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4-μHOM variant of EGFP
    Expression
    Mammalian
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113619-plasmid-map-sequence-id-218256

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  6. VEGFR2-YFP

    Plasmid
    #108853
    Purpose
    Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker
    Depositor
    Kalina Hristova
    Publication
    Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KDR (KDR Human)
    Tags
    Labeled with eYFP on the C-Terminus via a 3 amino…
    Expression
    Mammalian
    Available since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    108853-plasmid-map-sequence-id-208009

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  11. pIRES-hrGFP II-TET1_CD

    Plasmid
    #83570
    Purpose
    Expresses the catalytic domain of TET1 in mammalian cells
    Depositor
    Jean-Pierre Issa
    Publication
    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    Tags
    3X FLAG tag and hr GFP II
    Expression
    Mammalian
    Mutation
    TET1 amino acids 1-1417 deleted
    Promoter
    CMV
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83570-plasmid-map-sequence-id-191046

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  12. GST-mDia1(FH1FH2DAD)-His

    Plasmid
    #85822
    Purpose
    contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.
    Depositor
    Antoine Jégou, Guillaume Romet-Lemonne
    Publication
    Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc
    Insert
    Protein diaphanous homolog 1 (Diaph1 Mouse)
    Tags
    8*His and GST
    Expression
    Bacterial
    Promoter
    tac
    Available since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85822-plasmid-map-sequence-id-183287

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  15. hZMPSTE24-3FLAG_pQCXIP

    Plasmid
    #89759
    Purpose
    Human ZMPSTE24 mammalian expression vector. Retroviral bicistronic puromycin vector.
    Depositor
    Martin Dorf
    Publication
    Fu et al J Exp Med. 2017 Feb 28. pii: jem.2016127
    Insert
    Homo sapiens zinc metallopeptidase STE24 (ZMPSTE24) (ZMPSTE24 Human)
    Use
    Retroviral; Bicistronic expression: puromycin re…
    Tags
    9bp spacer (NotI site) - 3xFLAG epitopes - Stop c…
    Expression
    Mammalian
    Mutation
    Y253H in ZMPSTE24 (see depositor comments below)
    Promoter
    CMV
    Available since
    June 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89759-plasmid-map-sequence-id-180605

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  19. pcDNA-mCherry-4A/4B-CAAX

    Plasmid
    #87699
    Purpose
    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells
    Depositor
    Robert Campbell
    Publication
    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-4A/4B-CAAX
    Expression
    Mammalian
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    87699-plasmid-map-sequence-id-177046

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Showing: 9061 - 9080 of 66847 results