Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,278 results for: nes

Clear keywords
Showing: 9081 - 9100 of 16278 results

  1. TP1320

    Plasmid
    #252367
    Purpose
    Expression construct for an ALFA-sfGFP-SUMOEu anti-EMC nanobody with C-terminal His10 tag
    Depositor
    Tino Pleiner
    Publication
    Stanton et al bioRxiv [Preprint]. 2026 Jan 15:2026.01.
    Insert
    anti-EMC nanobody E2
    Tags
    ALFA-sfGFP-SUMOEu and His10
    Expression
    Bacterial
    Mutation
    Contains engineered nanobody variant with ALFA ta…
    Available since
    April 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    252367-plasmid-map-sequence-id-504519

    Enlarge

  2. p11_AaC60αβ/C20

    Plasmid
    #229506
    Purpose
    Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20
    Depositor
    Laura Gunn
    Publication
    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.
    Inserts
    Chaperonin 60α
    Chaperonin 60β
    Chaperonin 20
    Expression
    Bacterial
    Mutation
    N terminus chloroplast transit peptide truncation
    Promoter
    T7
    Available since
    June 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229506-plasmid-map-sequence-id-456756

    Enlarge

  3. pT/CAGGS-NRASG12V/D38A-IRES-mVenus

    Plasmid
    #236077
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).
    Depositor
    Masashi Narita
    Publication
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V D38A (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Mutation
    G12V D38A
    Promoter
    CAGGS
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236077-plasmid-map-sequence-id-472893

    Enlarge

  4. pJL NarL YdfI Flag

    Plasmid
    #225650
    Purpose
    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Publication
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NarL-YdfI (narL Synthetic)
    Use
    Cell free expression
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    NarL with native DNA-binding domain replaced with…
    Promoter
    T7
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    225650-plasmid-map-sequence-id-454890

    Enlarge

  7. KIF5A-GFP-SspB

    Plasmid
    #214401
    Purpose
    Expresses fusion of Kinesin heavy chain 5A (1-572) with GFP and SspB (micro)
    Depositor
    Liting Duan
    Publication
    Liu et al Proc Natl Acad Sci U S A. 2024 Apr 2;121
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF5A-GFP-SspB (Kif5a Synthetic, Rat)
    Tags
    KIF5A-GFP-SspB
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214401-plasmid-map-sequence-id-425805

    Enlarge

  8. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Publication
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

    Enlarge

  10. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

    Enlarge

  13. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161816-plasmid-map-sequence-id-320046

    Enlarge

  14. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159861
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Publication
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Expression
    Worm
    Mutation
    Not applicable
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159861-plasmid-map-sequence-id-318036

    Enlarge

  17. D5_Ld_280600

    Plasmid
    #135821
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Publication
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    major surface protease gp63, putative,leishmanolysin, putative
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    135821-plasmid-map-sequence-id-274515

    Enlarge

Showing: 9081 - 9100 of 16278 results