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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 32,166 results for: nes

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Showing: 9081 - 9100 of 32166 results

  1. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    86611-plasmid-map-sequence-id-168847

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  2. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid
    #86611
    Purpose
    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    86611-plasmid-map-sequence-id-168847

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  3. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  4. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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  13. pAR-9.47_VP2_AS

    Plasmid
    #74241
    Purpose
    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV9.47-VP2_AS
    Use
    AAV
    Tags
    19 alanines fused to VP2 of AAV9.47
    Available Since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74241-plasmid-map-sequence-id-141992

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  14. pAR-9.47_VP2_AS

    Plasmid
    #74241
    Purpose
    Original plasmid described in Choudhury et al, 2015 Mol. Ther. for production of AAV-AS vectors. Encodes VP2_AS chimeric protein with 19 alanines fused to the N-terminus of AAV9.47 VP2.
    Depositor
    Miguel Sena-Esteves
    Article
    Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2
    Inserts
    AAV2 Rep
    AAV9.47-VP2_AS
    Use
    AAV
    Tags
    19 alanines fused to VP2 of AAV9.47
    Available Since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    74241-plasmid-map-sequence-id-141992

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  19. TRMPV-ns

    Plasmid
    #27991
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Article
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TRMPV-ns (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available Since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    27991-plasmid-map-sequence-id-13246

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  20. TRMPV-ns

    Plasmid
    #27991
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Article
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TRMPV-ns (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available Since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    27991-plasmid-map-sequence-id-13246

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Showing: 9081 - 9100 of 32166 results