We narrowed to 20,324 results for: Sele
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Plasmid#187459PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-CALCOCO1
Plasmid#137765PurposeExpression and localization of tagged CALCOCO1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCALCOCO1 (CALCOCO1 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 13, MG-26-82)
Plasmid#110746PurposeBacterial expression of human USP30 (construct 13)DepositorInsertUSP30 (USP30 Human)
TagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350S, I353E + insertion deletion: 64-178;…PromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB309
Plasmid#68637PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTags3xFLAGExpressionPlantAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS1021
Plasmid#59575PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection markerDepositorPublicationInsertTwin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable sinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGS1022
Plasmid#59576PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection markerDepositorPublicationInsertTwin-Strep tag optimized for PCR amplification followed by URA3 selection marker
UseE. coli cloning vectorTagsTwin-Strep tagPromoterNoneAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
M3499 ura3::ADE2 Disruptor Converter
Plasmid#51674PurposeYeast marker swap plasmid for converting URA3 to ADE2DepositorPublicationAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpCas9-CBE6a-V106W
Plasmid#215824PurposeExpress CBE6a-V106W (with SpCas9) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCBE6a V106W-SpCas9-2xUGI (cas9 )
UseIn vitro transcription templateAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS1
Plasmid#175755PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1DepositorInsertLGALS1 (LGALS1 Human)
UseLentiviralAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-ALMS1
Plasmid#136877PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to CLIP-tagDepositorInsertCLIP-ALMS1 (ALMS1 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-BirA-eCPX-AP
Plasmid#121907PurposeExpresses BirA and eCPX-APDepositorInsertsTags6xHisExpressionBacterialPromoterT7 and pBAD promoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB336
Plasmid#68664PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB326
Plasmid#68654PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterNo PromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS1229
Plasmid#29115PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle203 (SLC7A5 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-E1.1-NRSE-RFP
Plasmid#22929DepositorInsertE1.1 binding site from Scardigli et al. 2003, with neuron-restrictive silencing element, with minimal CMV
UseAAVExpressionMammalianAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSSRB274_pAC8-eGFP-hsCRBN
Plasmid#218791PurposeInsect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
TagsFLAG-TEV-eGFP-PrescissionExpressionInsectMutationeGFP fused to N-terminusPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCR2 (CXCR2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin
Plasmid#211471Purposelentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistanceDepositorInsertCas9_T2A_mNeonGreen_P2A_BlasticidinR
UseCRISPR and LentiviralTags3xFlagExpressionMammalianPromoterhU6, EF-1aAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_VIP1.0
Plasmid#208682PurposeExpresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
ExpressionMammalianPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-CAAX
Plasmid#208683PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only