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PLRG1

Plasmid

#155827

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PLRG1 (PLRG1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3f3a KO sgRNA

Plasmid

#186940

Purpose

H3f3a KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3a KO sgRNA (H3f3a Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3f3b KO sgRNA

Plasmid

#186941

Purpose

H3f3b KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b KO sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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RK5F-YAP8SA

Plasmid

#125008

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

YAP8SA (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pPLN-hFluc-DM-eGFP-KDEL

Plasmid

#177728

Purpose

DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFlucDM

Tags

KDEL, Prolactin Signal Sequence, and eGFP

Expression

Bacterial and Mammalian

Mutation

R188Q, R261Q (Destabilized Mutant)

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pZA71

Plasmid

#174785

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker

Tags

3xFLAG

Expression

Plant

Mutation

WT

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pZA66

Plasmid

#174779

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

D481V

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pZA67

Plasmid

#174780

Purpose

In planta gene expression

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker

Tags

eGFP

Expression

Plant

Mutation

L17E

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shACO1

Plasmid

#169884

Purpose

Suppress ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

shRNA against ACO1

Use

Lentiviral and RNAi

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR1A_mmFbxw7a (closed)

Plasmid

#160101

Purpose

Expresses murine Fbxw7 alpha in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway Cloning

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human APC-B,C (1133–1189)

Plasmid

#132630

Purpose

expresses human APC-B,C (1133–1189) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

Insert

APC-B,C (1133–1189)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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SPIB_pet28a

Plasmid

#131655

Purpose

Bacterial expression of ETS gene SPIB

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPIB (SPIB Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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FB012

Plasmid

#119709

Purpose

Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC:hph:Ttub

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKK-mVenus-TEV

Plasmid

#105774

Purpose

Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mVenus-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pD765 FDFT1 3'UTR

Plasmid

#105765

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

FDFT1 3'UTR (FDFT1 Human)

Use

Luciferase

Mutation

None

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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E0040m (GFP)_CD

Plasmid

#66032

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - GFP

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pES001 (pQacA-deGFP)

Plasmid

#69030

Purpose

Reporter Plasmid - deGFP driven by a qacR responsive promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

deGFP

Expression

Bacterial

Promoter

qacA

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-CD151-7

Plasmid

#57358

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD151 (CD151 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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ReaLiSM A1-Hinge-GPIba+2cys

Plasmid

#59581

Purpose

express VWF A1-GPIba ReaLiSM construct in mammalian cells

Depositor

Timothy Springer

Article

Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

Insert

VWF A1-linker-GPIba (VWF Human)

Tags

6His

Expression

Mammalian

Promoter

chick beta-actin

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
M26V DJ-1 pET15b

Plasmid

#60688

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26V DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26V

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only