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non-targeting control gRNA (BRDN0001146476)

Plasmid

#80194

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001147797)

Plasmid

#80204

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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ULK2 gRNA (BRDN0001147288)

Plasmid

#77262

Purpose

3rd generation lentiviral gRNA plasmid targeting human ULK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ULK2 (ULK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ROR1 gRNA (BRDN0001162245)

Plasmid

#76043

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROR1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROR1 (ROR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK1 gRNA (BRDN0001147568)

Plasmid

#75522

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK1 (VRK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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VRK1 gRNA (BRDN0001148483)

Plasmid

#75523

Purpose

3rd generation lentiviral gRNA plasmid targeting human VRK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

VRK1 (VRK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pluc-miR148a-148aT

Plasmid

#65053

Purpose

Reporter plasmid to detect miR148/152 family expression

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

Engineered target sites miR-148a-3p (MIR148A Human, Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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STK33 gRNA (BRDN0001149402)

Plasmid

#76984

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK33

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK33 (STK33 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FH95pUp95bGW (B5)

Plasmid

#74014

Purpose

lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion and Psd95 shRNA

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA (Dlg4 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGY466

Plasmid

#166661

Purpose

For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox

Expression

Yeast

Mutation

Cre: E340A, D341A

Promoter

MET17

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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RIPK3 gRNA (BRDN0001148666)

Plasmid

#76999

Purpose

3rd generation lentiviral gRNA plasmid targeting human RIPK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RIPK3 (RIPK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRIM27 gRNA (BRDN0001148850)

Plasmid

#77517

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM27

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM27 (TRIM27 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK-GST-mEKLF

Plasmid

#67836

Purpose

Encodes GST fusion to murine EKLF (nucleotides 71-1215)

Depositor

James Bieker

Article

Zhang et al Proc Natl Acad Sci U S A. 1998 Aug 18;95

Insert

EKLF (Klf1 Mouse)

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
AAV-EF1α1.1-SomArchon-GFP

Plasmid

#153531

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LLP251 pEF1a-UNC5C-4-SunTag

Plasmid

#100940

Purpose

TALE vector with UNC5C-4 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE target sequence for UNC5C, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1P MUTYH_2

Plasmid

#160786

Purpose

Suppress MUTYH

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shMUTYH_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P MUTYH_1

Plasmid

#160785

Purpose

Suppress MUTYH

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shMUTYH_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001148852)

Plasmid

#80175

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
non-targeting control gRNA (BRDN0001147812)

Plasmid

#80176

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SRPK1 gRNA (BRDN0001147971)

Plasmid

#77492

Purpose

3rd generation lentiviral gRNA plasmid targeting human SRPK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SRPK1 (SRPK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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