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pAAV.CAG.(cyto).iGlucoSnFR2

Plasmid

#244110

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM106B-EGFP

Plasmid

#210366

Purpose

Expresses TMEM106B fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2

Plasmid

#244111

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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CoV2-N-WT-Hu1

Plasmid

#177937

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Hs.cargo(Cas9)

Plasmid

#174864

Purpose

Expresses human Peg10 cargoRNA encoding SpCas9

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

SpCas9 flanked by HsPeg10 UTRs

Tags

HA-SV40 NLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS

Plasmid

#86677

Purpose

Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

EGFP-NLS

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

Plasmid

#50942

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6s

Use

AAV

Promoter

hSyn1

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC18

Plasmid

#231666

Purpose

Expresses human ZDHHC18 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC18 (ZDHHC18 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC13

Plasmid

#231661

Purpose

Expresses human ZDHHC13 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC13 (ZDHHC13 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC14

Plasmid

#231662

Purpose

Expresses human ZDHHC14 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC14 (ZDHHC14 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC20

Plasmid

#231668

Purpose

Expresses human ZDHHC20 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC20 (ZDHHC20 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC12

Plasmid

#231660

Purpose

Expresses human ZDHHC12 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC12 (ZDHHC12 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC19

Plasmid

#231667

Purpose

Expresses human ZDHHC19 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC19 (ZDHHC19 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC4

Plasmid

#231653

Purpose

Expresses human ZDHHC4 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC4 (ZDHHC4 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC15

Plasmid

#231663

Purpose

Expresses human ZDHHC15 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC15 (ZDHHC15 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC23

Plasmid

#231671

Purpose

Expresses human ZDHHC23 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC23 (ZDHHC23 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC2

Plasmid

#231512

Purpose

Expresses human ZDHHC2 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC2 (ZDHHC2 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

Plasmid

#115219

Purpose

Expresses PspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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CDK1as_T2A_puromycin

Plasmid

#118595

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Tags

GFP

Expression

Bacterial and Yeast

Promoter

PRC1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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