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pENTR-2B-Nkx2-5 delta HD-G8

Plasmid

#98614

Purpose

Gateway entry vector NKX2-5 _HD

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 _HD (Nkx2-5 Mouse)

Use

Gateway Cloning

Mutation

Deletion of the homeodomain. Replaced by 8 glycin…

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1941

Plasmid

#82564

Purpose

High copy number plasmid containing Ple264 (NR2E1 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple264

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV6 CHIP delTPR-Myc

Plasmid

#246737

Purpose

Mammalian expression of human CHIP with TPR domain deleted, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

deletion of TPR domains

Promoter

CMV

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-VPS4A E228Q

Plasmid

#246736

Purpose

Mammalian expression of human VPS4A E228Q (dominant negative form) with a HA tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

VPS4A E228Q (DN) (VPS4A Human)

Tags

HA

Expression

Mammalian

Mutation

E228Q

Promoter

CMV

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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FSW CHIP delTPR-Myc

Plasmid

#246722

Purpose

Neuron-specific expression of human CHIP with TPR domain deleted, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

deletion of TPR domains

Promoter

Synapsin

Available Since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-GCaMP6s

Plasmid

#241023

Purpose

An HDR plasmid for the AAVS1 safe harbor locus carrying a gene cassette consisting of GCaMP6s under control of a CAG promoter and a WPRE.

Depositor

Thomas Johnson

Article

Aguzzi et al Sci Transl Med. 2026 Apr 29;18(847):eadr

Insert

GCaMP6s and WPRE

Use

CRISPR

Tags

6x His Tag

Promoter

CAG

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pKG01701_CMV-rtTA-betaglobin_pA-CMV-PuroR-bGH

Plasmid

#252540

Purpose

Secondary PiggyBac vector for puromycin selection and expression of transcriptional activator

Depositor

Kate Galloway

Article

Johnstone et al Science. 2026 Apr 30;392(6797):eadw1925.

Insert

rtTA

Expression

Mammalian

Promoter

CMV (human cytomegalovirus immediate early enhanc…

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pmMBD1.2G (pc2845)

Plasmid

#246849

Purpose

Mammalian expression plasmid of mouse MBD1 N-terminus (aa396-636). C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

Insert

MBD1 (Mbd1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pmGMBD1.3(pc2899)

Plasmid

#246850

Purpose

Mammalian expression plasmid of mouse MBD1 lacking the CXXC3 domain. N-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

Insert

MBD1 (Mbd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Mbd1ΔCXXC3

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pGmMBD1.4 (pc3305)

Plasmid

#246851

Purpose

Mammalian expression plasmid of mouse MBD1 lacking the MBD domain (aa 75–636). N-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

Insert

MBD1 (Mbd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Mbd1ΔMBD, aa 75–636

Promoter

CMV

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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