Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 18,343 results for: Mut

Showing: 9161 - 9180 of 18343 results

  1. EFSp-GFP-YAP (5SA)

    Plasmid
    #174170
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    EFS
    Available Since
    Sept. 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174170-plasmid-map-sequence-id-343725

    Enlarge

  2. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

    Enlarge

  3. AIMTOR A757

    Plasmid
    #140829
    Purpose
    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR A757 (ULK1 Human)
    Use
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Promoter
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140829-plasmid-map-sequence-id-305143

    Enlarge

  5. pDONR223_HSD17B10_WT_V5

    Plasmid
    #82941
    Purpose
    Gateway Donor vector containing HSD17B10, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    David Root, Matthew Meyerson, Jesse Boehm
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    HSD17B10 (HSD17B10 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82941-plasmid-map-sequence-id-162421

    Enlarge

  7. pEGFP-N1-HSD17B11-S172L-FLAGC

    Plasmid
    #161904
    Purpose
    Expresses the catalytically inactive S172L mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.
    Depositor
    Sébastien Britton
    Article
    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:
    Insert
    hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
    Use
    Tags
    FLAG-GFP
    Expression
    Mammalian
    Mutation
    S172L; catalytically dead mutant
    Promoter
    CMV
    Available Since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161904-plasmid-map-sequence-id-320523

    Enlarge

  12. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid
    #183078
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    V600E (T>A), S607S (TCC>AGT)
    Promoter
    None for primary insert. PGK for puromycin resist…
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183078-plasmid-map-sequence-id-363420

    Enlarge

  15. Target Accelerator Genetic Pathway Reference Set

    Plasmid Kit
    #1000000105
    Purpose
    Collection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processes
    Depositor
    Jesse Boehm
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. Ekker Lab TALEN kit

    Plasmid Kit
    #1000000038
    Purpose
    Contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs for high-throughput mutagenesis
    Depositor
    Stephen Ekker
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pENTR/pTER shATM #2 (730-2)

    Plasmid
    #22527
    Depositor
    Eric Campeau
    Insert
    sh Ataxia telangiectasia mutated
    Use
    RNAi; Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 17, 2009
    Availability
    Academic Institutions and Nonprofits only
    22527-plasmid-map-sequence-id-10357

    Enlarge

  18. pKP1392

    Plasmid
    #167649
    Purpose
    Express ExbD T42C protein
    Depositor
    Kathleen Postle
    Insert
    exbD T42C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbD with T42C mutation
    Promoter
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167649-plasmid-map-sequence-id-337289

    Enlarge

  19. pGM190oriT

    Plasmid
    #115679
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    oriT, aphII, tsr
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tipA
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    115679-plasmid-map-sequence-id-410983

    Enlarge

  20. rpoS200-RFP

    Plasmid
    #63840
    Purpose
    This plasmid is designed as a bacterial stationary phase sensor
    Depositor
    Howard Salis
    Insert
    rpoS, mRFP1 and GFPmut3b
    Use
    Tags
    mRFP1
    Expression
    Bacterial
    Mutation
    Gene is translationally fused to RFP
    Promoter
    Available Since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    63840-plasmid-map-sequence-id-106563

    Enlarge

Showing: 9161 - 9180 of 18343 results