Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

5'UTR stem-loop luciferase reporter: 0kcal/mol

5'UTR stem-loop luciferase reporter: -30kcal/mol

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

For targeted tethering of the MBD domain of human MBD6 using dCas13

Donor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]

An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.

tissue-specific multiplex gRNA plasmid

Gateway 5’ entry vector with the slc1a3b promoter

gRNA vector for AsCpf1

expression of moxMaple3 localized to the Golgi

expression plasmid of AsCpf1

Measurements of translational accuracy

Measurements of translational accuracy

Measurements of translational accuracy

E. coli expression vector for S. pyogenes Csn2-MBP-His

E. coli expression vector for His-MBP-S. pyogenes Cas1