We narrowed to 2,122 results for: D10

1. pCfb13220

   Plasmid

   #219895

   Purpose

   The base plasmid of TUNEYALI for TF30

   Depositor

   Irina Borodina

   Insert

   Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPD1101 Integration Test Circuit Vector

   Plasmid

   #139251

   Purpose

   Integration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.

   Depositor

   Joshua Leonard

   Insert

   Tus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE3G-VP64-dCas9-VP64-ZsG

   Plasmid

   #192668

   Purpose

   Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   VP64-dCas9-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   TRE3G

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMC00025 (aka pCW-dCas9-Blast)

   Plasmid

   #195316

   Purpose

   Dox inducible lentiviral dead Cas9

   Depositor

   Raj Chari

   Insert

   dead Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   D10A; H840A

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EF1alpha-dCas9-KRAB_Hygro

   Plasmid

   #192663

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUbi_dCas9-BFP-KRAB-gRNA

   Plasmid

   #221503

   Purpose

   CRISPRi machinery fish codon optimized expressing plasmid

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Tags

   Expression

   Mutation

   D10A and H839A point mutations

   Promoter

   Zebrafish Ubiquitin promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-ABE7.10-P2A-Puro

   Plasmid

   #112674

   Purpose

   Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

   Depositor

   Lukas Dow

   Insert

   ABE7.10
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. NM-01

   Plasmid

   #69799

   Purpose

   Bacterial expression of Cas9 with His, MBP, and Strep tags

   Depositor

   Gerard Cagney

   Insert

   Cas9
   

   Use

   Tags

   HIS, MBP, and StrepII

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

9. WT_H1-S1

   Plasmid

   #218400

   Purpose

   3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intron

   Depositor

   Oded Regev

   Insert

   Modified CHO DHFR minigene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetracycline-responsive promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. WT_S1-H1

    Plasmid

    #218392

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MUT_S1-N

    Plasmid

    #218398

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MUT_N-H1

    Plasmid

    #218397

    Purpose

    3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. WT_S7-H1

    Plasmid

    #218393

    Purpose

    3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetracycline-responsive promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid

    #192664

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUHD Cdk1 WT HA

    Plasmid

    #27652

    Depositor

    Greg Enders

    Insert

    Cdk1 (CDK1 Human)
    

    Use

    Tags

    HA (YDVPDYA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid

    #192667

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3991

    Plasmid

    #198149

    Purpose

    Mammalian expression of LAMP1

    Depositor

    Michael Hensel

    Insert

    PCMV::LAMP1::HaloTag::GFP (LAMP1 Human)
    

    Use

    Tags

    HaloTag-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. XE183 rat Frizzled-2-pCD

    Plasmid

    #16813

    Depositor

    Randall Moon

    Insert

    frizzled-2 (Fzd2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only