We narrowed to 42,524 results for: Eras
-
Plasmid#208966PurposeUnfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)
Plasmid#208974PurposeRecruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)
Plasmid#208973PurposeRecruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)
Plasmid#208971PurposeRecruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertPhi29-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(-exo;D169A)PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro
Plasmid#189634PurposeLentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs librariesDepositorInserthU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
UseCRISPR and LentiviralExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-Llgl1-WPRE-UbC-Emerald
Plasmid#203805PurposeLentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorAvailable SinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase
Plasmid#191481PurposeLuciferase reporter for SARS-CoV-2 5' UTR ΔSL1DepositorInsertSARS-CoV-2 5' UTR ΔSL1
UseLentiviral and LuciferaseMutationMissing the stem-loop 1 region of SARS-CoV-2 5…Available SinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut2x
Plasmid#182254PurposeHuman PDAP1 3` UTR region mutated in 2x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 2 binding sites for mir-150
UseLuciferaseExpressionMammalianAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut1x
Plasmid#182253PurposeHuman PDAP1 3` UTR region mutated in 1x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 1 binding site for mir-150
UseLuciferaseExpressionMammalianAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut3x
Plasmid#182255PurposeHuman PDAP1 3` UTR region mutated in 3x binding site for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of PDAP1 gene mutated in 3 binding sites for mir-150
UseLuciferaseExpressionMammalianAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA
Plasmid#216320PurposeSplit luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).DepositorInsertSplit Luciferase + splice donor site
UseAAVPromoterCMVAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3XFLAG-renilla-luciferase-beta-globin-control
Plasmid#184393PurposeTransient transfection plasmid expressing Renilla-luciferase-beta-globin fusion proteinDepositorInsertRenilla luciferase
Tags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)
Plasmid#184394PurposeTransient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globinDepositorInsertRenilla luciferase
Tags3X FLAGExpressionMammalianPromoterCMVAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE
Plasmid#236241PurposeLentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmeraldDepositorAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro CCND2-T2A-luciferase-P2A-eGFP
Plasmid#179843PurposeTunable and temporal expression control of CCND2, luciferase and nuclear eGFPDepositorInsertCCND2 (CCND2 Human)
UseLuciferase and Synthetic BiologyTagseGFP and luciferaseExpressionMammalianPromoterTRE3GAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)
Plasmid#242572PurposeGateway 3' entry clone containing an IRES LuciferaseDepositorInsertIRES-Luciferase-WPRE-bGH-pA
UseGateway CloningAvailable SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-myc-RRBP1-WPRE-UbC-Emerald
Plasmid#225942PurposeLentiviral vector plasmid expressing human ribosome binding protein 1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsmyc-tagExpressionMammalianPromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE
Plasmid#236239PurposeLentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmeraldDepositorAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald
Plasmid#225949PurposeLentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralExpressionMammalianMutationC100APromoterSFFV and UbCAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)
Plasmid#229840PurposeA Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.DepositorInsertLuciferase
ExpressionMammalianAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only