Addgene

We narrowed to 28,784 results for: GRN;

Showing: 901 - 920 of 28784 results

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HUWE1-sgRNA-2

Plasmid

#86925

Purpose

To express sgRNA target HUWE1 gene

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

HUWE1 sgRNA2 (HUWE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only
pUC57kan-T7-gRNA-U6 V2

Plasmid

#115520

Purpose

To construct multiple sgRNA expression vector for CRISPR

Depositor

Xingxu Huang

Insert

sgRNA scaffolf-U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pH-STEME-1-esgRNA

Plasmid

#138135

Purpose

Targeted simultaneous C-to-T and A-to-G in rice

Depositor

Caixia Gao

Insert

APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry_sgRNA-ver2

Plasmid

#126776

Purpose

gRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

sgRNA (for SpCas9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP73_sgRNA-1

Plasmid

#88855

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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TP53-KO_gRNA_1

Plasmid

#195130

Purpose

TP53-targeting gRNA in the LRG2.1 backbone

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

TP53 KO gRNA 1 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2_LSEC:Cas9green; erbb2_gRNA171

Plasmid

#199338

Purpose

pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

LSEC
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEMPTY::sgRNA2

Plasmid

#165459

Purpose

Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

Depositor

Inigo Lasa

Article

Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

Insert

sgRNA2

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

SP01

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-AAVS1_hspCas9

Plasmid

#193309

Purpose

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pH-STEME-NG-esgRNA

Plasmid

#138136

Purpose

Targeted simultaneous C-to-T and A-to-G in rice

Depositor

Caixia Gao

Insert

APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pU6a:sgRNA(tyr)

Plasmid

#64250

Purpose

expresses sgRNA(tyr) under U6a promoter

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

U6a:sgRNA (tyr)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

Plasmid

#187459

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-cj-E sgRNA

Plasmid

#169915

Purpose

Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA 2 GAPDH

Plasmid

#83809

Purpose

Express Sg-2 sgRNA targeting GAPDH

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

SgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pH-PABE-7-esgRNA

Plasmid

#115620

Purpose

Targeted A to G in rice

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-3*NLS

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_Psyn-sgRNA500

Plasmid

#149613

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-H1-gRNA

Plasmid

#61089

Purpose

CRISPR/Cas9 system gRNA cloning

Depositor

Shenglin Huang

Article

Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

Insert

H1 promoter; gRNA sequences

Use

CRISPR; /cas9 grna cloning vector

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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