We narrowed to 148,092 results for: MPI
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Plasmid#212136PurposeCargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_CbFDH
Plasmid#212140PurposeCargo plasmid for integrating PT7 expression cassette of formate dehydrogenase in genome. Plasmid can be removed by incubating cells at 37 C.DepositorInsertformate dehydrogenase
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPINA7A2mTFP1(F)
Plasmid#193774PurposeExpresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertmTFP1
UseSynthetic BiologyExpressionBacterialPromoterPINA7 synthetic promoter carrying binding sites f…Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-sfGFP-pA-FRT-Amp-FRT
Plasmid#176620Purposefor generating PCR templates with sfGFP and Amp for BAC recombinationDepositorInsertsuper folder green fluorescent protein with FRT-flanked ampicillin cassette
UseUnspecifiedMutationH217R - please see dep. commentsAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
ARK1a
Plasmid#81016PurposeE.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)DepositorInsertsMevTo-BBa1002-pTrc-Mkco-PMKco
PMD-NudB
PromoterLacUV5 and Trc promoterAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_GB
Plasmid#78677PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_FB
Plasmid#78676PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_EB
Plasmid#78675PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS2-pBAD_AB
Plasmid#78674PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS1-pBAD_AB
Plasmid#78670PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_I13453 to adjust spacing in MoClo system. [A:36INS1-pBAD:B]DepositorInsertInsulated pBAD Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
p416GAL1-Skn7t-L-IFP-F[1] (Yeast)
Plasmid#52896PurposeSkn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.DepositorInsertSkn7t-L-IFP-F[1]
TagsL-IFP-F[1]ExpressionYeastPromoterGAL1Available SinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX1 Chromo
Plasmid#59697PurposeContains histone modification interacting domain (CBX1 Chromo)DepositorAvailable SinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide
Plasmid#117986PurposepLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.DepositorInsertsgRNA backbone (F+E)
UseLentiviralAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
S12.2_Bridge + Target
Plasmid#127671PurposeAmpicillin resistant multiplexing backbone with CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsHAExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS_366
Plasmid#182131Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutationDepositorInsertsretron RNA
ec.86 RT
CspRecT
Mutationincorporated rifampicin-resistance-conferring don…Available SinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSNA
Plasmid#135192PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pASPIre2
Plasmid#154843PurposeDerivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1DepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMy-BirA-T2A-eGFP
Plasmid#50565PurposeRetroviral vector encoding for GFP and the biotinylating enzyme BirADepositorInsertAmpicilin
UseRetroviralAvailable SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYB2 reporter
Plasmid#197276PurposeEncodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYB2 and HaloTag (Vamp2 Rat)
UseLentiviralTagsFLAGMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only