We narrowed to 36,618 results for: Nes

1. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

   Plasmid

   #198054

   Purpose

   All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

   Depositor

   Lacra Bintu

   Article

   Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

   Inserts

   rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
   3xFLAG-insert
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G and pEF

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

   Plasmid

   #125234

   Purpose

   Expresses SPARK oβ2AR transmembrane component in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5
   

   Use

   AAV

   Tags

   Flag, V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIN-PAmCherry-KFERQ-NE

   Plasmid

   #102365

   Purpose

   Lentiviral overexpression of PA-mCherry-KFERQ fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   KFERQ peptide
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSIN-PAmCherry-mSNCA-NE

   Plasmid

   #102364

   Purpose

   Lentiviral overexpression of PA-mCherry synuclein alpha fusion

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Inserts

   PA-mCherry
   synuclein alpha (Snca Mouse)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ECFP-Bcr NE/AA

   Plasmid

   #36418

   Depositor

   Nora Heisterkamp, John Groffen

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   breakpoint cluster region (BCR Human)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   N689A/ E690A

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

6. EYFP-Abr NE/AA

   Plasmid

   #36419

   Depositor

   Nora Heisterkamp, John Groffen

   Article

   Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

   Insert

   active BCR-related (ABR Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   N245A, E246A

   Promoter

   Available since

   Aug. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

7. FUW-hSNCA(D2R)-NE

   Plasmid

   #215487

   Purpose

   Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE tag

   Expression

   Mammalian

   Mutation

   D (second aa) mutated to R

   Promoter

   Ubc

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-hSNCA(D2E)-NE

   Plasmid

   #215488

   Purpose

   Lentiviral expression of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE tag

   Expression

   Mammalian

   Mutation

   D (second aa) mutated to E

   Promoter

   Ubc

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-nE-mHP1alpha-delCTE

   Plasmid

   #181896

   Purpose

   Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   nE-mHP1alpha-delCTE (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPHR-mCherry-nE-mHP1alpha

    Plasmid

    #181890

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSIN-PAmCherry-mSNCA-NE

    Plasmid

    #102364

    Purpose

    Lentiviral overexpression of PA-mCherry synuclein alpha fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    synuclein alpha (Snca Mouse)
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ECFP-Bcr NE/AA

    Plasmid

    #36418

    Depositor

    John Groffen, Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    breakpoint cluster region (BCR Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    N689A/ E690A

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

14. EYFP-Abr NE/AA

    Plasmid

    #36419

    Depositor

    John Groffen, Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    active BCR-related (ABR Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    N245A, E246A

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. C. elegans miniMos transgenesis kit

    Plasmid Kit

    #1000000031

    Purpose

    29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.

    Depositor

    Erik Jorgensen

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pPyCAG-NE-mKate2-IRES-Hph

    Plasmid

    #183616

    Purpose

    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

    Depositor

    Sally Lowell

    Article

    Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

    Insert

    mKate2
    

    Use

    Tags

    Human EMD transmembrane domain and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPyCAG-NE-EGFP-IRES-Pac

    Plasmid

    #183617

    Purpose

    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

    Insert

    EGFP
    

    Use

    Tags

    Human EMD transmembrane domain and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPHR-mCherry-nE-mHP1alpha-delCTE

    Plasmid

    #181891

    Purpose

    Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-nE-mHP1alpha-delCTE (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Bcr DH domain NE/AA pGEX

    Plasmid

    #38180

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    BCR DH domain N689A/E690A (BCR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    BCR DH domain N689A/E690A

    Promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Abr DH domain NE/AA pGEX

    Plasmid

    #38182

    Depositor

    Nora Heisterkamp

    Article

    Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

    Insert

    ABR DH domain N282AE283A (ABR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    ABR DH domain with N282A and E283A mutations

    Promoter

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only