Purpose
pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
Insert
Upstream & Downstream pyrE deletion region
Use
E. coli - c. difficile shuttle vector
Tags
Available since
Oct. 17, 2019
Availability
Academic Institutions and Nonprofits only