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Plasmid#186396PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
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pENT-L1-Kozak-Venus-stop-L2
Plasmid#186360PurposeEntry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequencesDepositorInsertVenus yellow fluorescent protein
UseExpression of the venus fluorescent proteinTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK039
Plasmid#105425PurposeSynthetic biologyDepositorInsertdTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK038
Plasmid#105426PurposeSynthetic biologyDepositorInsertdTALE (binding to Bs3 promotor (Capsicum annuum) as transactivator)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI039
Plasmid#105428PurposeSynthetic biologyDepositorInsertLexA-VP16-ER (transcription factor: inducible expression; estradiol)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG822
Plasmid#105430PurposeSynthetic biologyDepositorInsertYFP fused to SV40 Nuclear Localisation Signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG587
Plasmid#105431PurposeSynthetic biologyDepositorInsertbeta-glucuronidase (2 introns; no ATG-Start codon)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM16418
Plasmid#105404PurposeSynthetic biologyDepositorInsert6xHis-NES(Rev)-GFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM16429
Plasmid#105405PurposeSynthetic biologyDepositorInsert6xHis-NES(PKI)-GFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG317
Plasmid#105407PurposeSynthetic biologyDepositorInsert4xmyc tag fused to Twin-Strep tag
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG659
Plasmid#105410PurposeSynthetic biologyDepositorInsertLuciferase CDS fused to 3xHA tag
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG643
Plasmid#105411PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG173
Plasmid#105417PurposeSynthetic biologyDepositorInsertmEGFP (GFP variant)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG172
Plasmid#105418PurposeSynthetic biologyDepositorInsertmTRQ (GFP variant)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG171
Plasmid#105419PurposeSynthetic biologyDepositorInsertmVenus (GFP variant)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG021
Plasmid#105420PurposeSynthetic biologyDepositorInserthpt (hygromycin phosphotransferase)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG351
Plasmid#105421PurposeSynthetic biologyDepositorInsertnptII (neomycin phosphotransferase II)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG031
Plasmid#105422PurposeSynthetic biologyDepositorInsertAvrRps4 Pseudomonas syringae pv. pisi)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG213
Plasmid#105385PurposeSynthetic biologyDepositorInsertpromotor SlEDS1 (Solyc060g071280; 1200bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG004
Plasmid#105386PurposeSynthetic biologyDepositorInsertpromotor SlEDS1 (Solyc060g071280; 1200bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only