We narrowed to 973 results for: nanobody

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mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Plasmid

#165029

Purpose

Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

Plasmid

#165026

Purpose

Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

Plasmid

#165028

Purpose

Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

Depositor

Yubin Zhou

Article

Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

Insert

LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

T7

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_NbNP_mStayGold_GB1

Plasmid

#242816

Purpose

Stable expression of NbNP-mStayGold

Depositor

Marvin Tanenbaum

Article

Rabouw et al Cell Systems, 2026; 17

Insert

NbNP

Use

Lentiviral

Tags

mStayGold

Expression

Mammalian

Promoter

SFFV

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 5HT2A_NB2_1

Plasmid

#127690

Purpose

Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

5HT2A_NB2

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b-LaG9(S151C)

Plasmid

#172490

Purpose

Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

Depositor

Siegfried Musser

Article

Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

Insert

LaG9(S151C)

Tags

6xHis, PPX cleavage site, and pelB leader sequenc…

Expression

Bacterial

Mutation

S151C

Promoter

T7

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

Plasmid

#223536

Purpose

Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Insert

LaG16_synNotch_tTA

Use

Lentiviral

Tags

c-myc tag

Expression

Mammalian

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-Nb37

Plasmid

#205514

Purpose

Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-Nb37

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-Halo (MoonTag-Nb-Halo)

Plasmid

#128603

Purpose

Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and Halo

Promoter

SFFV

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Nb-gp41-GFP (MoonTag-Nb-GFP)

Plasmid

#128602

Purpose

Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Insert

Nanobody_gp41

Use

Lentiviral

Tags

GB1 and sfGFP

Promoter

SFFV

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pVDL9.3

Plasmid

#168299

Purpose

Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

Depositor

Víctor de Lorenzo

Article

Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

Insert

Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 JDM16-GFPnb-mCh

Plasmid

#235667

Purpose

Expresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherry

Depositor

David Baker

Article

Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

Insert

JDM16-GFPnb-mCh (Mch Synthetic)

Tags

GFP nanobody and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-NbNP-AausFP1-GB1-NLS

Plasmid

#242815

Purpose

Stable expression of NbNP-AausFP1 with NLS

Depositor

Marvin Tanenbaum

Article

Rabouw et al Cell Systems, 2026; 17

Insert

NbNP

Use

Lentiviral

Tags

AausFP1

Expression

Mammalian

Promoter

SFFV

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-CD5-NbHA-mCherry

Plasmid

#242817

Purpose

Stable expression of NbHA-mCherry for secretion of fusion protein into the extracellular medium

Depositor

Marvin Tanenbaum

Article

Rabouw et al Cell Systems, 2026; 17

Insert

NbHA

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-CD5-NbHA-sfGFP

Plasmid

#242818

Purpose

Stable expression of NbHA-superfolderGFP for secretion of fusion protein into the extracellular medium

Depositor

Marvin Tanenbaum

Article

Rabouw et al Cell Systems, 2026; 17

Insert

NbHA

Use

Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

SFFV

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_886

Plasmid

#201162

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-Nb80

Plasmid

#205518

Purpose

Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-Nb80

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_888

Plasmid

#201165

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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