We narrowed to 5,532 results for: pcas

1. pCAG-WHaloCaMP1a-EGFP

   Plasmid

   #205313

   Purpose

   Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-CBE6b-V106W

   Plasmid

   #215825

   Purpose

   Express CBE6b V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9 TadCBEd-V106W

   Plasmid

   #193839

   Purpose

   Express TadCBEd-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd V106W-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-loxPSTOPloxP-ZsGreen

   Plasmid

   #51269

   Purpose

   Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   loxPSTOPloxP-ZsGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-HIF1 alpha

   Plasmid

   #21101

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRSV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-loxSTOPlox-LynEGFP

   Plasmid

   #133922

   Purpose

   Reporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombination

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   LynEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-memRFP-3xControlgRNA

   Plasmid

   #224567

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCap-101-Apr

   Plasmid

   #227507

   Purpose

   Capture plasmid with apramycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCap-SG5-Apr

    Plasmid

    #227579

    Purpose

    Capture plasmid with apramycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCap-101-Hgy

    Plasmid

    #227508

    Purpose

    Capture plasmid with hygromycin resistance and pIJ101 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCap-SG5-Hgy

    Plasmid

    #227580

    Purpose

    Capture plasmid with hygromycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-opCas12a-6xNLS

    Plasmid

    #209022

    Purpose

    Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R

    Promoter

    EF-1a

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG/ATP10D(E215Q)-HA

    Plasmid

    #209248

    Purpose

    Mammalian expression of ATP10D mutant E215Q

    Depositor

    Hye-Won Shin

    Article

    Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

    Insert

    ATP10D (ATP10D Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E215Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-NLS-HA-Bxb1

    Plasmid

    #51271

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    NLS-HA-Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. SB100X in pCAG globin pA

    Plasmid

    #127909

    Purpose

    Sleeping Beauty Transposase expression vector driven by the CAG promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    Sleeping Beauty Transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EFS-SpCas9

    Plasmid

    #104588

    Purpose

    AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

    Depositor

    Ryohei Yasuda

    Article

    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

    Insert

    Streptococcus pyogenes Cas9
    

    Use

    AAV and CRISPR

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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