We narrowed to 41,696 results for: tro

1. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

   Plasmid

   #168253

   Purpose

   "label stable F-actin; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Utrophin-GFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

   Plasmid

   #172477

   Purpose

   sfGFP with a synthetic intron derived from the human beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

   Plasmid

   #172478

   Purpose

   sfGFP with a synthetic intron from the pCI expression vector (Promega)

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

   Plasmid

   #172475

   Purpose

   sfGFP with a synthetic intron derived from the mouse Ighe gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CAG-loxP-3xpA-loxP-sfGFP+RbG syntron

   Plasmid

   #172476

   Purpose

   sfGFP with a synthetic intron derived from the rabbit beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128347

   Purpose

   pAAV encoding control gRNA for CRISPR gRNAs listed above

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   control (negative)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

   Plasmid

   #235087

   Purpose

   GFP splicing reporter with FRG1 intron

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   custom bidirectional

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

   Plasmid

   #235086

   Purpose

   GFP splicing reporter with CORO1B intron

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   custom bidirectional

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

   Plasmid

   #184395

   Purpose

   Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Firefly luciferase beta-globin
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

    Plasmid

    #184396

    Purpose

    Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

    Plasmid

    #48283

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSF4 CMV 5TOP intron renilla TRICK CTE polyA

    Plasmid

    #64544

    Purpose

    5′ TOP TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    12x PP7 stem-loops
    

    Use

    Tags

    24xMS2 stem loops, 5' TOP (5′ terminal oligo…

    Expression

    Mammalian

    Mutation

    Promoter

    Tetracycline-inducible promoter and 5′ UTR of hum…

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid

    #119189

    Purpose

    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.

    Depositor

    Alex Dunn

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    

    Use

    Transposon

    Tags

    mTFP1-F40-mEYFP

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    TCE

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid

    #119188

    Purpose

    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.

    Depositor

    Alex Dunn

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    

    Use

    Transposon

    Tags

    mTFP1-F40-mEYFP

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    TCE

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid

    #208943

    Purpose

    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid

    #172479

    Purpose

    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

    Plasmid

    #208945

    Purpose

    A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

    Plasmid

    #159866

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid

    #118717

    Purpose

    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry(Y72L)

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge