We narrowed to 19,051 results for: Tec

1. pINTO-NFH::mEzh2

   Plasmid

   #65925

   Purpose

   Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Insert

   Enhancer of zeste homolog 2 (Ezh2 Mouse)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV/TO (T-REx) and CMV/TetO2 (T-REx)

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. RPS6KA2 gRNA (BRDN0001147334)

   Plasmid

   #75732

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA2 (RPS6KA2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307-SARS-CoV-3CL

   Plasmid

   #160280

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Agarabi CHO Heavy Chain in pJ201

   Plasmid

   #111567

   Purpose

   Cloning vector containing CDS for the modified human heavy chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

   Depositor

   Cyrus Agarabi

   Article

   Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

   Insert

   Human heavy chain
   

   Use

   Synthetic Biology; Cloning vector

   Promoter

   None

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHRIG-PRAS40

   Plasmid

   #53599

   Purpose

   lentiviral expression of constitutively active PRAS40 and EGFP

   Depositor

   Heng Zhao

   Article

   Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

   Insert

   PRAS40 (AKT1S1 Human)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP and myc

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

7. PKN1 gRNA (BRDN0001162558)

   Plasmid

   #76278

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKN1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKN1 (PKN1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGV3319

   Plasmid

   #170277

   Purpose

   Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   Bst-LF D720A
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   D720A

   Promoter

   T7-lac

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30a-His6-ABP-LOXCATmut

   Plasmid

   #139972

   Purpose

   A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

   Depositor

   Vamsi Mootha

   Article

   Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Insert

   A fusion of lactate oxidase from A. viridans and catalase from E. coli
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutation in H265A and R268A in LOX (used original…

   Promoter

   T7

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAPM-miR30-EIF2AK2-ts1

    Plasmid

    #174247

    Purpose

    EIF2AK2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    EIF2AK2 shRNA (EIF2AK2 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSHUT4-eYFP

    Plasmid

    #133094

    Purpose

    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

    Depositor

    Jens Laurids Sørensen

    Article

    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

    Insert

    enhanced yellow flourescent protein
    

    Use

    Fungal expression

    Promoter

    TEF-1α

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRB112

    Plasmid

    #180173

    Purpose

    pAAV construct GluN1WT (shRNA resistant)

    Depositor

    Richard Bergeron

    Article

    Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

    Insert

    GluN1 (Grin1 Rat)
    

    Use

    AAV

    Tags

    Phluorin

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV/myc/ER-αT2ib

    Plasmid

    #110769

    Purpose

    Expresses ER intrabody against mouse and human TLR2

    Depositor

    Thomas Böldicke

    Article

    Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

    Insert

    intrabody against mouse and human TLR2
    

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PGK1 gRNA (BRDN0001144730)

    Plasmid

    #77980

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PGK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PGK1 (PGK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. hA3A-BE3 (pRZ215)

    Plasmid

    #131314

    Purpose

    CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PIKFYVE gRNA (BRDN0001147303)

    Plasmid

    #76892

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIKFYVE

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIKFYVE (PIKFYVE Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_POLD2-APOBEC1-UdgX-NG-nCas9-UdgX

    Plasmid

    #163545

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-NG-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. PGK1 gRNA (BRDN0001487049)

    Plasmid

    #77981

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PGK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PGK1 (PGK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PITX3-2A-eGFP-PGK-Puro

    Plasmid

    #31943

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    PITX3 (PITX3 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Promoter

    -

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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