166,787 results

1. Human Metabolic Gene CRISPRa sgRNA library

   Pooled Library

   #187080

   Purpose

   Lentiviral CRISPRa library targeting human metabolic genes for activation of gene expression

   Depositor

   Kivanc Birsoy

   Article

   Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

2. mTagBFP2-PCNA-19-SV40NLS-4

   Plasmid

   #55320

   Purpose

   Localization: Nucleus, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   3xSV40 NLS and mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-EF1a-Cre

   Plasmid

   #55636

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV11

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)

   Viral Prep

   #100850-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 (#100850). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Tags

   None

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pMpGE014

   Plasmid

   #108682

   Purpose

   All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

   Depositor

   Takayuki Kohchi, Shigeo Sugano

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-SopF

   Plasmid

   #135174

   Purpose

   Expresses an mCherry-SopF fusion in mammalian cells

   Depositor

   Leigh Knodler

   Article

   Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

   Insert

   SopF (STM1239 Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-Hb9-GFP

   Plasmid

   #37080

   Purpose

   3rd generation lentiviral transfer plasmid for Hb9 GFP

   Depositor

   Fred Gage

   Article

   Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

   Insert

   Hb9 GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Hb9

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pECE

   Plasmid

   #26453

   Depositor

   William Rutter

   Article

   Ellis et al Cell. 1986 Jun 6;45(5):721-32.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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