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pEMS1964

Plasmid

#82574

Purpose

High copy number plasmid containing Ple305 MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple305

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 23, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_PIK3R1-1/1

Plasmid

#91274

Purpose

Protein expression and purification of human SH3 domain construct PIK3R1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PIK3R1-1/1 (PIK3R1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P13L

Plasmid

#177946

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P13L) (N )

Expression

Mammalian

Mutation

P13L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P80R

Plasmid

#177947

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P80R) (N )

Expression

Mammalian

Mutation

P80R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-S235F

Plasmid

#177956

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S235F) (N )

Expression

Mammalian

Mutation

S235F

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2453 - [1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

Plasmid

#159863

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal(900bp PATCs, NLS, no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_NEDD9-1/1

Plasmid

#91498

Purpose

Protein expression and purification of human SH3 domain construct NEDD9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NEDD9-1/1 (NEDD9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW-GFP-2-cut reporter

Plasmid

#131669

Purpose

Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

GFP-2-cut reporter

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHL 1917

Plasmid

#53024

Purpose

AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

mCherry
fimB (fimB E. coli)

Use

Synthetic Biology

Tags

Asp terminator, RBS(st7), and T1 terminator

Expression

Bacterial

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B-NNQ-HA-GFP

Plasmid

#121164

Purpose

Expresses mmPtch1-B NNQ variant

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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