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pRS303-PADH1-AFB2

Plasmid

#99530

Purpose

S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)

Depositor

Helle Ulrich

Insert

AFB2 (AFB2 Mustard Weed)

Expression

Yeast

Promoter

ADH1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSP6-EBNA(2A+DBD)

Plasmid

#98749

Purpose

In vitro transcription of modified EBNA1 mRNA

Depositor

Sara Howden, Jim Vadolas

Article

Howden et al Hum Gene Ther. 2006 Aug;17(8):833-44.

Insert

Modified EBNA1

Use

In vitro transcription

Promoter

SP6

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pFS462

Plasmid

#89065

Purpose

his7 integration plasmid with Z3EVpr:GFP

Depositor

Nick Rhind

Article

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Insert

Z3EV promoter GFP-NLS leu1 C-terminal 300 nt

Use

S. pombe his7 integration vector

Promoter

Z3EV

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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Px461-Cas9n-Trp53-sgRNA-alpha

Plasmid

#88846

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53 (Trp53 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pDONR223_EGFR_p.ELREA_746del

Plasmid

#82911

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

Deletion of residues 746-750 (ELREA)

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G13D

Plasmid

#82808

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G13D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-Skp2

Plasmid

#89142

Purpose

mammalian expression of human Skp2 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

Insert

Skp2 (SKP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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UAS-3xHalo7::CAAX

Plasmid

#87646

Purpose

For creating drosophila transgenics expressing UAS-3xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

3xHalo7::CAAX

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-VSV-G-Myc

Plasmid

#80054

Purpose

Expression of a Myc tagged VSV-G protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VSV-G-Myc

Tags

Myc

Expression

Mammalian

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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p1xNLS-FRB-mCherry-hsp86-BSD

Plasmid

#85795

Purpose

Expression of 1xNLS mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

1xNLS Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Promoter

Pfhsp86

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP-Vpr-RR

Plasmid

#83374

Purpose

Expression of Myc tagged GFP-Vpr fusion protein

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP-Vpr

Tags

Myc and Myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTH1

Plasmid

#84451

Purpose

shuttle plasmid for sarA P1-dsRED expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

DsRed.T3(DNT)

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRN10

Plasmid

#84454

Purpose

shuttle plasmid for sarA P1-mKate expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mKate2

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

Plasmid

#86157

Purpose

FLS2-GFP expresssion

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

3xmyc and GFP

Expression

Plant

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

Plasmid

#84244

Purpose

Expresses GA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sa dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM10-HA

Plasmid

#65107

Purpose

Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM10 (ADAM10 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 19-455

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

Plasmid

#67991

Purpose

CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette

Use

CRISPR and Lentiviral

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-H63D

Plasmid

#82036

Purpose

Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Tags

HA

Expression

Mammalian

Mutation

Exonuclease-dead mutant: H63D MRE11

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB101

Plasmid

#68555

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-wtGATA6-3XAU1 neo

Plasmid

#72618

Purpose

Expresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Promoter

TRE Tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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