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eGFP-ova-C1

Plasmid

#163524

Purpose

Expresses both eGFP and chicken ovalbumin in mammalian cells

Depositor

Thomas Weber

Insert

enhanced-GFP-chicken ovalbumin tandem

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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P22_wtCP_SP141his

Plasmid

#122651

Purpose

Expression of P22 virus like particle in bacteria

Depositor

Trevor Douglas

Insert

P22 Coat: WT / SP: 6xHis-SP141

Expression

Bacterial

Mutation

truncated SP, P120H in the capsid scaffold (see …

Available Since

Aug. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Prrx1:TFPnls-T2a-Cre-Ert

Plasmid

#111150

Purpose

Limb connective tissue specific inducible Cre

Depositor

Elly Tanaka

Insert

TFPnls-T2a-Cre-Ert

Use

Cre/Lox

Mutation

wt

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SiriusGFP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJK266

Plasmid

#73326

Purpose

Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

Depositor

T. J. Kappock

Insert

violacein biosynthesis protein VioD

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP Asn10 TEV LIC cloning vector (1C)

Plasmid

#29654

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xAsn, 6xHis, MBP, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR11 CA NFAT1

Plasmid

#11792

Depositor

Anjana Rao

Insert

NFAT1 (Nfatc2 Mouse)

Tags

HA

Expression

Bacterial

Mutation

Constitutively active: multiple serine to alanine…

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA3-hCD2

Plasmid

#21810

Depositor

David Ron

Insert

CD2 (CD2 Human)

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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RedTrackCMV

Plasmid

#50957

Purpose

A backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFP

Depositor

James Bamburg

Type

Empty backbone

Use

Adenoviral

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Pax8

Plasmid

#11994

Depositor

Steven Johnson

Insert

PAX8 (PAX8 Human)

Expression

Mammalian

Mutation

The following construct contains the PAX8 promote…

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR ARRET

Plasmid

#111384

Purpose

ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Insert

ARRET

Use

Gateway entry cloning

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-RSV-jGCaMP7f-W

Plasmid

#126691

Purpose

Mammalian calcium sensor protein.

Depositor

Darrell Kotton

Insert

jGCaMP7f

Use

Lentiviral

Tags

6xHIS, T7, Xpress

Mutation

H78K, A317L

Promoter

RSV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-NSP3SA11-F2A-EGFP

Plasmid

#220808

Purpose

Express separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996)

Depositor

Alex Borodavka

Insert

Rotavirus SA11 NSP3-F2A-EGFP

Use

Other

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6MFV SLC27A2

Plasmid

#194136

Purpose

Expresses FATP2 (SLC27A2) protein in mammalian cells

Depositor

Jun Liu

Insert

SLC27A2 (SLC27A2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-MT2A

Plasmid

#11613

Depositor

Steven Johnson

Insert

metallothionein 2A (MT2A Human)

Tags

GFP

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR5-CFP

Plasmid

#13019

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 5 (TLR5 Human)

Tags

CFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-hIL7-IRES-ZsGreen-W

Plasmid

#26532

Depositor

David Baltimore

Insert

IL7 (IL7 Human)

Use

Lentiviral

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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SIRT6

Plasmid

#28271

Depositor

Cheryl Arrowsmith

Insert

SIRT6 (SIRT6 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Available Since

Feb. 10, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pOpen-K12polLF (exo-)

Plasmid

#165522

Purpose

DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)

Use

Synthetic Biology

Available Since

Dec. 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Rich-pT7

Plasmid

#108445

Purpose

This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

Depositor

Richard Bélanger

Type

Empty backbone

Promoter

pT7

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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