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RFP-(SUMO FAAA)3

Plasmid

#130703

Purpose

Mammalian expression plasmid for negative control of mCherry-(SUMO)3.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

3 copies of human SUMO1 separated by (GGS)4

Expression

Mammalian

Mutation

FKVK sequence in each SUMO1 module changed to FAAA

Promoter

CMV

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-entry+NLS

Plasmid

#128013

Purpose

Vector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

Gal4 DBD-SV40NLS-3x-Flag

Use

Gal4 entry vector with nls to generate tethering …

Tags

Gal4-DBD-SV40NLS-3xFlag

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_266-341

Plasmid

#118821

Purpose

Bacterial expression of hnRNPA2 truncation

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His and TEV

Expression

Bacterial

Mutation

contains amino acids 266-341

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO2 ts2

Plasmid

#115854

Purpose

AGO2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO2 shRNA (AGO2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBiex1-SYNAC

Plasmid

#82710

Purpose

Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

Insert

SYNAC (ADCY2, ADCY5 Human)

Tags

FLAG, mCerulean, and mCitrine

Expression

Bacterial and Insect

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot10

Plasmid

#79254

Purpose

Expresses EGFP-tagged DmNot10 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot10 (Not10 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot11

Plasmid

#79253

Purpose

Expresses EGFP-tagged DmNot11 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot11 (Not11 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2-FDM3

Plasmid

#72210

Purpose

Mammalian expression of aa 227-860 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-226

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pRJPaph-eBFP2

Plasmid

#67030

Purpose

Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Insert

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR

Plasmid

#48157

Purpose

Transgenically expresses firefly luciferase protein in T. cruzi

Depositor

Rick Tarleton

Article

Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

Insert

luciferase

Use

Luciferase; T. cruzi expression

Promoter

T7

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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psiCheck-2-3 x mir-34

Plasmid

#40831

Purpose

Luciferase reporter

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

three sites partially complementary to miR-34 (mir-34 Fly)

Use

RNAi

Tags

luciferase

Expression

Insect

Promoter

SV40

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only
p6722 MSCV-IP N FlagHA 16E6 I128T

Plasmid

#44154

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

HPV16 E6 I128T mutant

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
p6721 MSCV-IP N FlagHA 16E6 8S9A10T

Plasmid

#44153

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

E6 8S9A10T mutant

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pENTR-MXC

Plasmid

#37140

Depositor

Robert Duronio

Article

White et al J Cell Biol. 2011 May 16;193(4):677-94.

Insert

Multi Sex combs (mxc Fly)

Use

Gateway Cloning

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
HL 818 (=HL 716 + pHL 98)

Plasmid

#30006

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLlacO-1:DsrA-mcherry, pLtetO-1:rpoS::gfp

Use

Synthetic Biology

Tags

mcherry, GFP

Expression

Bacterial

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-GAP1IP4BPdeltaC2

Plasmid

#20199

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 26;184(2):297-308.

Insert

GAP1IP4BP-detlaC2 (RASA3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of C2 domains

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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5' Del 1

Plasmid

#16049

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter, 5' deletion (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1372 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
5' Del 2

Plasmid

#16050

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter, 5' deletion (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1147 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pTEF-Sup35C

Plasmid

#1202

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35 C-terminus (SUP35 Budding Yeast)

Expression

Yeast

Mutation

Sup35 C-terminus ~254aa-end

Promoter

TEF

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pBT362

Plasmid

#127844

Purpose

clone BE4max-NG CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max-NG without deaminase

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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