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pL0_AC_pNub

Plasmid

#154038

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter Nub

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_EF_tSVP

Plasmid

#154041

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator SVP

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pL0_AC_pPbt

Plasmid

#154042

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter Pbt

Use

Synthetic Biology

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPSG1059

Plasmid

#158595

Purpose

tNCS-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Inserts

tNCS-ePTS1
Ec_CYP80B1
Ps_6OMT
Ps_4'OMT2
Ps_CNMT

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPSG1060

Plasmid

#158596

Purpose

tNCS-dead-ePTS1, 6OMT, 4'OMT2, CNMT (CEN6/ARS4)

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Inserts

tNCS-dead_ePTS1
Ec_CYP80B1
Ps_6OMT
Ps_4'OMT2
Ps_CNMT

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFast-dCry-V5His

Plasmid

#136240

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Insect

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSKAP

Plasmid

#122702

Purpose

Designed for the incorporation of DNA barcode sequences for subsequent allelic exchange on bacterial chromosomes.

Depositor

Travis Bourret

Article

Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37

Type

Empty backbone

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCuHA-SpAtg101(414)

Plasmid

#122081

Purpose

Expressing S. pombe HA-Atg101

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG101

Tags

3HA

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHMGWA-Cas1

Plasmid

#122285

Purpose

Cas1 cloned in pHMGWA

Depositor

Jennifer Doudna

Article

Nunez et al Nat Struct Mol Biol. 2014 Jun;21(6):528-

Insert

Eco Cas1

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPGK1-(AscI)-λN-T2A-SpHIS5 (pNTI347)

Plasmid

#115429

Purpose

Selection for in-frame fusion expression constructs

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Type

Empty backbone

Tags

T2A-SpHIS5 and λN

Expression

Yeast

Promoter

PGK1

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS1GG

Plasmid

#108197

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

S. solfataricus PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pS3GG

Plasmid

#108199

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

S. solfataricus PCNA3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pS2GG

Plasmid

#108198

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

S. solfataricus PCNA2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pM2GG

Plasmid

#108201

Purpose

M. sedula PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pM3GG

Plasmid

#108202

Purpose

M. sedula PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaG4SPdX

Plasmid

#108203

Purpose

G4S-PdX

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Putidaredoxin

Expression

Bacterial

Mutation

changed Cysteine 73 to Serine and Cysteine 85 to …

Promoter

lac promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sgd1

Plasmid

#100000

Purpose

Shuttle Sgd1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sgd1 (SGD1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Bms1

Plasmid

#100001

Purpose

Shuttle Bms1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Bms1 (BMS1 Budding Yeast)

Expression

Yeast

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb1

Plasmid

#99981

Purpose

Shuttle yeast Ckb1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb1 (CKB1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb2

Plasmid

#99982

Purpose

Shuttle yeast Ckb2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb2 (CKB2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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