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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 47,293 results for: crispr

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Showing: 9221 - 9240 of 47293 results

  4. pCMV-hA3A-eBE-Y130F

    Plasmid
    #113423
    Purpose
    Expresses hA3A-eBE-Y130F in mammalian cells
    Depositor
    Jia Chen
    Publication
    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    hAPOBEC3A_Y130F
    Promoter
    CMV
    Available since
    Aug. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113423-plasmid-map-sequence-id-217432

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  7. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid
    #140220
    Purpose
    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1
    Use
    CRISPR
    Tags
    miniVPR-mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140220-plasmid-map-sequence-id-291993

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  8. dead Sa Cas9

    Plasmid
    #138162
    Purpose
    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSaCas9
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D10A + N580A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    138162-plasmid-map-sequence-id-272344

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  12. pSLQ10875

    Plasmid
    #183963
    Purpose
    CBA-hyperdCas12a-HA-KRAB-mCherry
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, KRAB, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CBA
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183963-plasmid-map-sequence-id-363118

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  16. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    208942-plasmid-map-sequence-id-415667

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  17. dead Sa Cas9

    Plasmid
    #138162
    Purpose
    For orthogonal R loop assay (use with Sp-derived base editor, Sp gRNA, and Sa gRNA for assay)
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSaCas9
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    D10A + N580A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    138162-plasmid-map-sequence-id-272344

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Showing: 9221 - 9240 of 47293 results