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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,447 results for: grn

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Showing: 9221 - 9240 of 15447 results

  5. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Publication
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  6. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Publication
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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  9. tet-pLKO.puro_shHuR CDS

    Plasmid
    #110411
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    110411-plasmid-map-sequence-id-213311

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  13. pSLIK-Venus-TmiR-G13-G12

    Plasmid
    #25742
    Purpose
    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.
    Depositor
    Iain Fraser
    Publication
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Insert
    G alpha 13 and 12 miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    Venus
    Expression
    Mammalian
    Available since
    July 14, 2010
    Availability
    Academic Institutions and Nonprofits only
    25742-plasmid-map-sequence-id-12067

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  17. pspCas9-HF-tetra-com-vector

    Plasmid
    #132555
    Purpose
    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.
    Depositor
    Baisong Lu
    Publication
    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    Expression
    Mammalian
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    132555-plasmid-map-sequence-id-259534

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Showing: 9221 - 9240 of 15447 results