We narrowed to 19,051 results for: Tec

1. RIPK1 gRNA (BRDN0001149147)

   Plasmid

   #76535

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RIPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MLM3709

   Plasmid

   #49943

   Purpose

   Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   3x FLAG Tet1CD RH-3 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   3x FLAG

   Promoter

   EF1a

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. CCL2 gRNA (BRDN0001148290)

   Plasmid

   #77867

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CCL2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CCL2 gRNA (CCL2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-hA3A-eBE-Y132D

   Plasmid

   #113429

   Purpose

   Expresses hA3A-eBE-Y132D in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y132D

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCHKO_GFP_Luciferase

   Plasmid

   #155079

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PLK4 gRNA (BRDN0001146388)

   Plasmid

   #76234

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PLK4 (PLK4 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PI4K2A gRNA (BRDN0001147595)

   Plasmid

   #76822

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PI4K2A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PI4K2A (PI4K2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PDPK1 gRNA (BRDN0001146405)

   Plasmid

   #77453

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PDPK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PDPK1 (PDPK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLEX307-HCoV-NL63-3CL-C144A

   Plasmid

   #160287

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C144A

   Promoter

   EF1a

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCold I-Hero13

    Plasmid

    #187929

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero13

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero13 (BEX3 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLEX307-SARS-CoV-3CL-C145A

    Plasmid

    #160281

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NADK2 gRNA (BRDN0001487085)

    Plasmid

    #78093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NADK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NADK2 (NADK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PRKCQ gRNA (BRDN0001144886)

    Plasmid

    #75553

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCQ

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCQ (PRKCQ Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PRKCQ gRNA (BRDN0001145693)

    Plasmid

    #75554

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCQ

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCQ (PRKCQ Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX131

    Plasmid

    #167149

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    4'-phosphopantetheinyl transferase, npgA
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid

    #123339

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-EKAR-EV
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JA740

    Plasmid

    #49939

    Purpose

    Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in EGFP for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD GFP (TET1 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

18. OCT4 GFP puro Donor 3

    Plasmid

    #22211

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-OCT-GFP-2APuro-PA (POU5F1 Human)
    

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTOR gRNA (BRDN0001147813)

    Plasmid

    #76783

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MTOR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTOR gRNA (BRDN0001145082)

    Plasmid

    #76782

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MTOR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge