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pSB_init-Sb44

Plasmid

#159421

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb44

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

Plasmid

#60658

Purpose

Flp-dependent expression of mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Has Service

AAV8

Insert

FLEX-mNaChBac-T2A-tdTomato

Use

AAV

Promoter

EF1α

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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LifeAct-LSSmGFP

Plasmid

#186534

Purpose

Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to actin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP-Puro-TetOn-L1RP-ORF1_mNeonGreen2

Plasmid

#202566

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopy

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Tags

mNeonGreen2 on ORF1p

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPE4max-Blasticidin

Plasmid

#194905

Purpose

Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

Insert

BSD

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLP1

Plasmid

#209988

Purpose

Entry vector to store Level 0 parts

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGDP1 fosA

Plasmid

#112889

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

fosA

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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AAV.hSyn.GLPLight1

Plasmid

#187466

Purpose

Expresses GLPLight1 in neurons

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight1

Use

AAV

Tags

Flag tag

Promoter

human Synapsin-1

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1169 FIREmate-ECFP-Cb5

Plasmid

#205167

Purpose

Expresses FIREmate-ECFP-Cb5 in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

FIREmate-ECFP-Cb5

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_mVenus_Hyg

Plasmid

#240651

Purpose

Expression plasmid for TEV-mVenus-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGY199

Plasmid

#239801

Purpose

Express EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

EGFP

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3 GATA Luc

Plasmid

#85695

Purpose

Luciferase reporter containing three repeats of the GATA consensus site

Depositor

Licio Collavin, Giannino Del Sal

Article

Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

Insert

3xGATA and minimal MT promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

3xGATA and minimal MT promoter

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-ITGAM POD-nAb-WPRE

Plasmid

#244975

Purpose

Expresses a fusion protein of a nanobody against integrin alpha M (ITGAM; CD11b) and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

ITGAM POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ11434

Plasmid

#183625

Purpose

pHR-R(HRE-minCMV-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

Depositor

Stanley Qi

Article

Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

Insert

Cas12 Guide (crGFP*)

Use

CRISPR and Lentiviral

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-msfGFPBb

Plasmid

#118231

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mNeptune-pBAD

Plasmid

#54714

Purpose

Localization: Protein Expression Vector , Excitation: 600, Emission: 650

Depositor

Robert Campbell, Michael Davidson, Nathan Shaner, Roger Tsien

Article

Lin et al Chem Biol. 2009 Nov 25;16(11):1169-79. d

Type

Empty backbone

Tags

6xHis and mNeptune

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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MLV Gag-RFP

Plasmid

#1814

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

RFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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