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pDIV488

Plasmid

#177696

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_102-680_D

Plasmid

#146124

Purpose

Insect Expression of DmEDC3_102-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_102-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_331-680_D

Plasmid

#146125

Purpose

Insect Expression of DmEDC3_331-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_331-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Pigb-V5

Plasmid

#175160

Purpose

Lentiviral expression of mouse Pigb-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Pigb (Pigb Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mSpata33-PA

Plasmid

#179129

Purpose

Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

spermatogenesis associated 33 (Spata33 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTF

Plasmid

#162784

Purpose

Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

Depositor

Ivan Vorobiev

Article

Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

Type

Empty backbone

Tags

10xHis and FLAG

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+WBP2NL-HA

Plasmid

#174791

Purpose

Xenopus laevis WBP2NL with 3'HA tag

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

WBP2 N-terminal like (wbp2nl.L Frog)

Tags

HA

Expression

Bacterial

Mutation

aa#165, Y to H; aa# 287, D to H

Promoter

SP6

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2S31A

Plasmid

#174332

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-NKD2S31A

Plasmid

#174331

Purpose

encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

MycHis

Expression

Mammalian

Mutation

changed Serine 31 to Alanine

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-wbp2nl

Plasmid

#172463

Purpose

expresses wild-type protein when injected into embryos

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl (wbp2nl.L Frog)

Use

Xenopus expression

Tags

none

Mutation

none

Promoter

SP6

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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UUUUA-pHG165c

Plasmid

#170107

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

UUUUA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MMMMA-pHG165c

Plasmid

#170108

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

MelR

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA-pHG165c

Plasmid

#170110

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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XXXXA_Q182A_R287H-pHG165c

Plasmid

#170111

Purpose

Constitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

XXXXA_Q182A_R278H

Expression

Bacterial

Mutation

Q182A, R287H

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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RRRAA-pHG165c

Plasmid

#170112

Purpose

Constitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

RRRAA

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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SaLgCG

Plasmid

#164563

Purpose

GFP

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

Plasmid

#153195

Purpose

encodes pAAV NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAB-mOvol1

Plasmid

#120360

Purpose

piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

Ovol1 (Ovol1 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tet-on and CBh

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-3

Plasmid

#160703

Purpose

tef1-alpha promoter from Trichoderma virens bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

tef1-alpha

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 R14A/K310A

Plasmid

#162655

Purpose

Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids R14 and K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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