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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 9261 - 9280 of 18351 results

  1. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

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  3. pJin-290

    Plasmid
    #125718
    Purpose
    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19 with CGG RNAP-C
    Depositor
    Bryan Dickinson
    Publication
    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCGG-gRNA GFP-off expression, FKBP-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-FRB
    Expression
    Mammalian
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125718-plasmid-map-sequence-id-244348

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  4. pHH29_32 series plasmid

    Plasmid
    #120901
    Purpose
    Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device
    Depositor
    Domitilla Del Vecchio
    Publication
    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:
    Inserts
    Ec-TTL-P109
    sRNA-A targeting sequence
    RBS TIR=974 of GFPop1 gene
    GFPop1
    RBS TIR=6474 of ECF32mut gene
    ECF32mut
    T7 terminator
    resource competitor
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120901-plasmid-map-sequence-id-241429

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  7. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Publication
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  9. pSV40-GUS-P2A-Hygro

    Plasmid
    #106480
    Purpose
    Expression of GUS and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    beta-glucuronidase
    Hygromycin B resistance
    Expression
    Mammalian and Plant
    Promoter
    SV40 promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106480-plasmid-map-sequence-id-207984

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  10. pZyxin-mMaple2

    Plasmid
    #101150
    Purpose
    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate
    Depositor
    Xiaowei Zhuang
    Publication
    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized mMaple2 fused to zyxin
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    101150-plasmid-map-sequence-id-205974

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  14. PsbA2-HMGS-HMGR-ATOB-NPTI

    Plasmid
    #73338
    Purpose
    Construct expressing the upper MVA pathway in Synechocystis PCC 6803
    Depositor
    Anastasios Melis
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HMGS-HMGR-ATOB-NPTI
    Promoter
    psbA2
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73338-plasmid-map-sequence-id-135049

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  15. pJZC73

    Plasmid
    #62341
    Purpose
    sgRNA (no RNA aptamer addition) with COM-KRAB effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Publication
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: GAATAGCTCAGAGGCC…
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62341-plasmid-map-sequence-id-102264

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  16. pFrt-invCAG-Luc

    Plasmid
    #63577
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional Luciferase expression upon integration. The vector contains an FRT site and two mutant loxP sites
    Depositor
    Ivo Huijbers
    Publication
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Expression
    Mammalian
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    63577-plasmid-map-sequence-id-105728

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Showing: 9261 - 9280 of 18351 results