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pRK5-mEGFP-RAX-WT

Plasmid

#194572

Purpose

Mammalian Expression of mEGFP-RAX WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDNA5-FRT/TO-eGFP-TONSL

Plasmid

#221012

Purpose

mammalian expression vector of eGFP tagged TONSL siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

TONSL (TONSL Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

Promoter

CMV

Available Since

June 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pWZL Neo Myr Flag AKAP8L

Plasmid

#20481

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKAP8L (AKAP8L Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PCTK3

Plasmid

#20562

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PCTK3 (CDK18 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag BLK

Plasmid

#20430

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BLK (BLK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
AiP995-pAAV-mscRE10-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163485

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP995 - pAAV-AiE2010m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PIM1

Plasmid

#20579

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIM1 (PIM1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

PIM1 amino acids 312 & 313 are changed from S…

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPK7

Plasmid

#20527

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK7 (MAPK7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag HK3

Plasmid

#20414

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK3 (HK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP3K14

Plasmid

#20640

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K14 (MAP3K14 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
AiP1048-pAAV-mscRE10-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163481

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1048 - pAAV-AiE2010m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NLS

Plasmid

#133023

Purpose

NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag AURKA

Plasmid

#20427

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AURKA (AURKA Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TTK

Plasmid

#20659

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TTK (TTK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP3K8

Plasmid

#20521

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K8 (MAP3K8 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pRS426-HsFECH

Plasmid

#174524

Purpose

Expresses human ferrochelatase (FECH) in yeast

Depositor

Oleh Khalimonchuk

Article

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

FECH with yeast HEM15 promoter and terminator (FECH Human, Synthetic, Budding Yeast)

Expression

Yeast

Mutation

Lacks original mitochondrial targeting sequence (…

Promoter

HEM15 (yeast, 257 bp)

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha2-EGFP

Plasmid

#160976

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha3-EGFP

Plasmid

#160977

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag LIMK1

Plasmid

#20512

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LIMK1 (LIMK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

aa1-273 only

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

Plasmid

#216733

Purpose

Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

Depositor

Vincent Dion

Article

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase and sgCTG

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A

Promoter

U6 and CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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