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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,964 results for: nin

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Showing: 9321 - 9340 of 66964 results

  3. pCAG-mEGFP-CaMKIIa (T286A)

    Plasmid
    #127390
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)
    Depositor
    Ryohei Yasuda
    Publication
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Alanine
    Promoter
    pCAG
    Available since
    Jan. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    127390-plasmid-map-sequence-id-250429

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  4. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129027-plasmid-map-sequence-id-256129

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  5. pcDNA4/TO/Strep-HA-ZAKalpha_K45A

    Plasmid
    #141194
    Purpose
    Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line
    Depositor
    Simon Bekker-Jensen
    Publication
    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAP3K20 (MAP3K20 Human)
    Tags
    Strep, HA
    Expression
    Mammalian
    Mutation
    Changed Lysine 45 to Alanine
    Promoter
    CMV
    Available since
    June 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141194-plasmid-map-sequence-id-299516

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  6. pENTR_L1-miniTurbo-NES-YFP-STOP-L2

    Plasmid
    #127359
    Purpose
    gateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choice
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, NES, V5, and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127359-plasmid-map-sequence-id-246306

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  8. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid
    #51248
    Purpose
    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala
    Depositor
    Rajat Rohatgi
    Publication
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gli3 (Gli3 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV and Flag
    Expression
    Mammalian
    Mutation
    amino acids 849,865,877,907,980,1006 mutated to A…
    Promoter
    EF1a
    Available since
    March 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Publication
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  13. pTripZ RB1 WT-3XFLAG

    Plasmid
    #209070
    Purpose
    DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag
    Depositor
    Matthew Oser
    Publication
    Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do
    Insert
    human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag (RB1 RB1 WT, Human)
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    209070-plasmid-map-sequence-id-412296

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  14. SOX9-T2A-H2B-EGFP repair template

    Plasmid
    #167972
    Purpose
    SOX9 reporter repair template
    Depositor
    Emma Rawlins
    Publication
    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SRY-Box Transcription Factor 9 (SOX9 Human)
    Use
    CRISPR; Donor template
    Tags
    H2B-EGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167972-plasmid-map-sequence-id-331137

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  15. pDEST-CMV 3xFLAG-GABARAPL2-GFP

    Plasmid
    #123113
    Purpose
    Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123113-plasmid-map-sequence-id-244024

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  17. pCAG-GFP-PQRv3-RFP

    Plasmid
    #73951
    Purpose
    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.
    Depositor
    Brian Chen
    Publication
    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sfGFP (superfolder GFP)
    RFP
    Tags
    A residual PQR peptide wii be left at the C-termi…
    Expression
    Mammalian
    Promoter
    Chicken beta actin and Chicken beta actin (shared…
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only

  19. pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D

    Plasmid
    #177320
    Purpose
    Lentiviral vector expressing PAmCherry1-tagged KRas 4B G12D mutant, with an N-terminal dimerization domain (DD).
    Depositor
    Steven Chu, Xiaolin Nan
    Publication
    Nan et al Proc Natl Acad Sci U S A. 2015 Jun 30;11
    Insert
    KRAS 4B (KRAS Human)
    Use
    Lentiviral
    Tags
    FKBP-derived dimerization domain (DD, or DmrB) an…
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    TetOn-CMV
    Available since
    Jan. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    177320-plasmid-map-sequence-id-353281

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  20. pLM-AMA15.0 Cas9

    Plasmid
    #138944
    Purpose
    Fungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markers
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10
    Insert
    p40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
    Use
    CRISPR and Synthetic Biology; Ama1 autonomously r…
    Tags
    NLS
    Promoter
    p40S (AN0465) Aspergillus nidulans
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138944-plasmid-map-sequence-id-364383

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Showing: 9321 - 9340 of 66964 results