We narrowed to 59,251 results for: {{{s}}}

1. Limk1 gRNA#2

   Plasmid

   #163392

   Purpose

   Cas9-mediated knockout of Limk1 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Limk1 (Limk1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Eomes gRNA#2

   Plasmid

   #170835

   Purpose

   Cas9-mediated knockout of Eomes in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Eomes (Eomes Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Eomes gRNA#3

   Plasmid

   #170836

   Purpose

   Cas9-mediated knockout of Eomes in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Eomes (Eomes Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Grin1 gRNA#1

   Plasmid

   #169789

   Purpose

   Cas9-mediated knockout of Grin1 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAFNF-Venus-Actb

   Plasmid

   #169788

   Purpose

   An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pThuAsol

   Plasmid

   #171675

   Purpose

   pThuAsol expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   SUMO-TEV-ThuAsol
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

   Plasmid

   #168772

   Purpose

   Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   Hrasls (plaat1 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   alphaA-crystallin promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGST-Fah_S

   Plasmid

   #163088

   Purpose

   expressing mouse swingshift mutant FAH in bacterial cells

   Depositor

   Joseph Takahashi

   Article

   Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

   Insert

   Fah_S
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME-QUASM1:GFPNLS-SV40pA

   Plasmid

   #155116

   Purpose

   Middle entry vector containing QUASM1 element upstream of GFP-NLS.

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1:GFPNLS
   

   Use

   Gateway middle entry vector

   Tags

   Expression

   Mutation

   Promoter

   QUASM1-E1b

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pENTR-L3-Csy4-Cas9-EGFP-L2

    Plasmid

    #141045

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

    Plasmid

    #141051

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pENTR-L3-Csy4-FokI-dCas9-L2

    Plasmid

    #141042

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-FokI-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-L3-Cas9n-EGFP-L2

    Plasmid

    #141044

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9n-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-L3-Csy4-Cas9n-L2

    Plasmid

    #141041

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9n
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-L3-Cas9-EGFP-L2

    Plasmid

    #141043

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR-L3-SpCas9-HF4-2A-EGFP-L2

    Plasmid

    #141049

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF4-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-L3-SpCas9-HF2-2A-EGFP-L2

    Plasmid

    #141050

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF2-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-L3-Csy4-FokI-dCas9-EGFP-L2

    Plasmid

    #141047

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-FokI-dCas9-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-L3-Csy4-Cas9-L2

    Plasmid

    #141040

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge