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pHR- flag-Lag9-iCAM-1

Plasmid

#205209

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG9

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag9 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

Plasmid

#178883

Purpose

Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

U6-Nla repeat-BbsI-Nla repeat

Expression

Mammalian

Promoter

U6

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Human, Mouse)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

Plasmid

#62904

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD28 SNIPR

Plasmid

#188358

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP Rac2

Plasmid

#12193

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

Plasmid

#62951

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-LNGFR-FF3

Plasmid

#11666

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

Plasmid

#115209

Purpose

Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PbuCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

Plasmid

#62949

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-Intb2

Plasmid

#205203

Purpose

This vector encodes of the synCAM (Intb2) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Intb2 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp4

Plasmid

#163588

Purpose

Expresses BMP-4 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp4 (Bmp4 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Mllt3

Plasmid

#131604

Purpose

expression of mouse MIIt3 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Mllt3 (Mllt3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-SspBx6

Plasmid

#103778

Purpose

expression of iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

YFP-SspBx6

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO)3

Plasmid

#127150

Purpose

Mammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-OPHN1

Plasmid

#221310

Purpose

Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

OPHN1 (pLxIS) (Ophn1 Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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DsRed-p150 217–548

Plasmid

#51146

Purpose

Inhibits dynein-dynactin interaction in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 2002 Oct 28;159(2):245-54.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

Q118R, E340K

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only
pCF287_EFS-TiCas9-P2A-Puro

Plasmid

#211646

Purpose

EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Tamoxifen-inducible SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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